TUNEL staining assay was applied to evaluate neuronal cell apoptosis in the hippocampus after administration of miR-499-5p in HIE rat model. Neurobehavioral assays were conducted to evaluate the effect of miR-499-5p on the neurological functions of rat pups with HI-induced brain injury. Our ...
《miR-499-5p对猪肌纤维类型转化的调控及其机制研究》是黄志清为项目负责人，四川农业大学为依托单位的面上项目。项目摘要 肌纤维类型与猪肉品质密切相关。对比猪不同类型骨骼肌中miR-499-5p的表达水平，我们发现比目鱼肌（慢肌）显著高于趾长伸肌（快肌）。而Sox6的表达水平则相反。分析发现，猪Sox6 3'UTR中含有...
货号:mml-miR-499-5p 原价:￥4030 现价:￥3098.38 节省:￥932 规格:1 kits 说明:mml-miR-499-5p 特异性茎环引物试剂盒 ￥615产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方式与靶基因的29’UTR区部分或完全互补,剪切靶基因的转录产物或者抑制...
目的:研究miR-499-5p靶向调控钙通道电压依赖性β1蛋白(CACNβ1)在慢性心房颤动心房重构的作用.方法:构建心房颤动新西兰大白兔模型,动物实验分为sham组,模型组,对照组,实验组,在造模前48 h,对照组和实验组分别以30 mg/kg剂量心脏冠脉注射rAAV9-miR-499-5p-inhibitors-NC和rAAV9-miR-499-5p-inhibitors,sham...
The development of new therapeutic strategies based on the miR-499-5p-p21 axis is a promising path to overcome DOX cardiotoxicity as a chemotherapy for cancer treatment. 展开 关键词: doxorubicin, miR-499-5p, p21, cardiotoxicity, mitochondrial, apoptosis ...
同时转染200 nM的miR-499-5p inhibitor抑制miR-499-5p的表达.结果表明,不同添加剂量的PB2均可促进C2C12肌管MyHC I基因的表达(P<0.05);添加5μg/mL和10μg/mL的PB2显著增加猪肌管MyHC I基因的表达(P<0.05);添加5μg/mL和10μg/mL的PB2上调C2C12肌管和猪肌管miR-499-5p的表达水平(P<0.05).机制研究...
产品活性:hsa-miR-499a-5p antagomir 是一种经过特殊化学修饰的成熟miRNA的互补单链,全链进行甲氧基修饰,在5'端和3'端分别有2个和4个硫代骨架修饰,并在3'端连接有高亲和性胆固醇修饰。miRNA antagomir通过特异性结合成熟miRNA,阻止miRNA与其靶基因的互补配对,从而抑制miRNA的功能。相比于miRNA inhibitor,miRNA ant...
In addition, miR‐499a‐5p was down‐regulated meanwhile exerted tumour‐suppressive function in glioma cells. Subsequently, inhibition of SCAMP1 significantly restrained the cell proliferation, migration and invasion, as well as promoted apoptosis by acting as a molecular sponge of miR‐499a‐5p. ...
《miR-499-5p-FOXO4-miR-185轴调控结肠癌EMT和转移的机制研究》是依托中国人民解放军第四军医大学，由樊代明担任项目负责人的面上项目。中文摘要 转移是导致结肠癌预后不良的关键问题。上皮间质转化（EMT）是肿瘤转移的关键启动步骤，其在结肠癌进展中起主导作用。新近研究表明，miRNA在肿瘤EMT发生中发挥重...