(real-time fluorogenic quantitative-PCR,RFQ-PCR)检测移植瘤组织中miR-374b-5p的表达水平;荧光素酶活性检测验证miR-374b-5p是否靶向作用于RECK基因;分别采用RFQ-PCR和免疫组织化学法检测移植瘤中RECK mRNA和蛋白的表达情况.结果:miR-374b-5p抑制组移植瘤体积在转染miR-374b-5p inhibitor 9 d后(即接种细胞的第...
R-374b-5p expression with anti-mi R-374b-5p significantly suppressed the metastasis,invasion and proliferation of MGC-803 cells.The bioinformatic analysis predicted that the 3'untranslated region(UTR)of reversion-inducing cysteine-rich protein with Kazal motif(RECK)contains three mi R-374b-5p ...
hsa-miR-374b-5p antagomir CAS No. : MCE 国际站:hsa-miR-374b-5p antagomir 产品活性:hsa-miR-374b-5p antagomir 是一种经过特殊化学修饰的成熟miRNA的互补单链,全链进行甲氧基修饰,在5'端和3'端分别有2个和4个硫代骨架修饰,并在3'端连接有高亲和性胆固醇修饰。miRNA antagomir通过特异性结合成熟mi...
miR-374b-5p may be explored as a promising diagnostic marker and therapeutic target for DVT. Graphical abstract Download: Download high-res image (232KB) Download: Download full-size image Introduction Deep vein thrombosis (DVT) occurs when a thrombus forms in a vein, usually in the deep ...
ABCA8 was down-regulated by miR-374b-5p; this down-regulation can induce epithelial transformation to mesenchyme via the ERK/ZEB1 signaling pathway and promote HCC progression. Conclusion We exposed the prognostic value of ABCA8 in HCC, and illuminated a novel pathway in ABCA8-regulated ...
目的探讨非小细胞肺癌(NSCLC)患者血清微RNA-374b-5p(miR-374b-5p),血管内皮生长因子C(VEGF-C)的表达及与预后的关系.方法选取2018年2月至2019年9月山东省临沂市肿瘤医院(以下简称"我院")收治的105例NSCLC患者为NSCLC组,我院同期54名体检健康者为对照组.检测并比较两组血清miR-374b-5p与VEGF-C表达;TargetScan...
By analyzing the miRNA sequencing dataset of pancreatic cancer from The Cancer Genome Atlas, it was demonstrated that miR-374b-5p expression was dramatically reduced in pancreatic cancer tissues compared with adjacent normal tissues, as well as decreased in chemoresistant compared with chemosensitive ...
The expression of miR-374b-5p was measured by quantitative real-time polymerase chain reaction (qRT-PCR) in 78 paired PDAC tissue samples. The biological effects of miR-374b-5p were investigated using 展开 关键词: KDM5B epithelial-mesenchymal transition miR-374b-5p pancreatic cancer ...
《miR-374b-5p在黄芩素抑制人肝癌细胞增殖中的作用及分子机制》是依托西安交通大学，由别蓓蓓担任项目负责人的青年科学基金项目。中文摘要 黄芩素具有广泛的抗肿瘤活性，研究发现miRNA可参与中药的抗肿瘤效应，但其是否参与黄芩素的抗肿瘤作用还未见报道。本课题组前期采用miRNA芯片分析发现肝癌细胞中miR...