1、鉴定导致肌肉萎缩的miRNA:miRNA Array、去小腿右坐骨神经的小鼠模型、qRT-PCR等 2、miR-29b体外功能研究:miRNA mimics、过表达载体、miRNA inhibitors等 3、miR-29b作用机制:TargetScan、荧光素酶报告分析、siRNA、mimics、inhibitors等 4、miR-29b体内功能验证:miRNA agomir、HE染色、PAS与SDH染色、抗体免疫荧光染色...
MMP-2 is a target gene of miR-29b. The overexpression of miR-29b significantly downregulated the expression of MMP-2 mRNA and protein. miR-29b mimics inhibited the cell viability of SMCs, and cell apoptosis was significantly enhanced compared with the NC group, especially in the early stage....
方法将miR-29b模拟物(miR-29bmimics)和miR-29b抑制剂(miR-29binhibitors)转染至人胰腺癌5-氟尿嘧啶(5-fluorouracil,5-Fu)耐药细胞株Patu8988/5-Fu,采用qRT-PCR检测参芪扶正注射液(5、10、20 μL/mL)对Patu8988/5-Fu细胞miR-29b...
MiR-29b mimics promotes cell apoptosis of smooth muscle cells via targeting on MMP-2 Abstract The phenotypic transformation and dysfunctions of vascular smooth muscle cells (SMCs) such as abnormality proliferation and apoptosis are key pathological basis of atherosclerosis. The recent study aimed to det...
miR-29b通过靶向调节特异性蛋白1调控糖尿病性白内障晶状体上皮细胞间质转化的作用 欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁氉 氉 氉 氉 引文格式：牟琳，刘苹，李来，...
However, it should be taken into account that these effects represent the result of the whole miR-29b mimics activity on both MM cells and cells of the microenvironment compartment. Finally, we investigated the functional relevance of impaired IL-23 secretion. We co-cultured for 48 h 29b-DCs ...
The miR-29b mimics suppressed podocytes' inflammation mediated through macrophages and attenuated HDAC4 expression, glomerular damage and renal fibrosis. Conclusion This study concludes that miR-29b regulates the expression of HDAC4 which plays a role in controlling renal fibrosis and podocytes' ...
方法 将 miR-29b 模拟物(miR-29b mimics)和 miR-29b 抑制剂(miR-29b inhibitors)转染至人胰腺癌 5-氟尿嘧啶(5-fluorouracil,5-Fu)耐药细胞株 Patu8988/5-Fu,采用 qRT-PCR 检测参芪扶正注射液(5,10,20 μL/mL)对 Patu8988/5-Fu 细胞 miR-29b mRNA 表达的影响;采用 MTT 法检测 miR-29b mimics 和 ...
TNF-a蛋白相对含量明显降低,miR-29b相对表达量明显升高，心肌梗死面积百分比明显减小(P< 0.05)，心肌组织损伤减轻。与MA组比较,MR组STAT3、p-STAT3、IL-lB、TNF-a蛋白相对含量明显 升高,miR-29b相对表达量明显降低，心肌梗死面积百分比明显增大(P<0.05),心肌组织损伤加重。转染STAT3-3，UTR-WT、miR-29b mimics...
MiR-29b mimics and negative control miR were transfected by lipofection using RNAiMAX and the effects on the MMT evaluated in vitro. HPMC produced substantial amounts of miR-29b which was markedly inhibited by TGF-β1. TGF-β1 stimulation of HPMC induced morphological changes with decreased ...