In silico analyses showed TIM17A as a potential target of miR-133a (seed binding sequence: UUAGAAGAUAAAGGACCAA) and miRNA-mRNA EMSA revealed that miR-133a directly targets TIM17A (EMSA binding: WT 1.32±0.14 and mutant 0.40±0.22 using 3′UTR probes). In conclusion, we demonstrated that ...
The sequences of miRNA mimics and AMOs are showed in Table 2. Table 2 The sequences of miRNA mimics and AMOs Full size table Mimic and AMO of miRNA pretreatment in vivo Mimic and AMO of miRNA pretreatment in vivo were performed as previously described [22]. With the chest open as ...
Each row represents a single sequence. The bar graphs depict the methylation rates of all 5 CpG sites (B) and single CpG sites (C). Full size image In order to further determine the causal role of DNA methylation in the enlargement of cell size and perturbations of key regulators of ...
Moreover, both the number of the unique miRNA sequences and the amount of miRNA species were mildly lower in LOH group compared with control group (828 vs 1011, 2374833 vs 4984030, respectively) (Fig. 1c–f). The deep sequencing data and analyses of differentially expressed miRNAs were ...
ThemiR-133aas well asmiR-145had the target sequence ofFSCN1mRNA by the database search, and both microRNAs repressed the mRNA and protein expression of FSCN1. The luciferase assay revealed thatmiR-145andmiR-133awere directly bound toFSCN1mRNA. Cell viability was significantly inhibited inmiR-...
miRNA target sequences were inserted between the XhoI-PmeI restriction sites in the multiple cloning region in the 30UTR of the hRluc gene. The 30UTR region of FSCN1 contains 4 putative miR-145 recognition sites and 1 133a/b recognition site ampli?ed from the genomic DNA of cancer cells....
BC specimens were subjected to immunohistochemistry of FSCN1 and in situ hybridisation of miR-145.Results:The miR-133a as well as miR-145 had the target sequence of FSCN1 mRNA by the database search, and both microRNAs repressed the mRNA and protein expression of FSCN1. The luciferase ...
The sequences of primers used in the study were shown in Additional file 1: Table S1. All samples were measured in triplicate. Western blotting Cells were lysed in a lysis buffer (Cell Signaling Technology, USA) supplemented with protease inhibitors (Calbiochem, USA) at 4 °C for 30 min,...
These miRNAs (miR-145, miR-133a and miR-133b), which have conserved sequences in the 3'UTR of FSCN1 (actin-binding protein, Fascin homolog 1), inhibited FSCN1 expression. The signal from a luciferase reporter assay was significantly decreased at 2 miR-145 target sites and 1 miR-133a/...
Their expression is controlled by serum response factor (SRF), MEF2, and MyoD transcription factors, which bind to sequences located upstream from the miRNAs and/or to their intergenic sequences (Liu et al., 2008, Zhao et al., 2005). Overexpression of miR-1 mediates cardiomyocyte withdrawal ...