In silico analyses showed TIM17A as a potential target of miR-133a (seed binding sequence: UUAGAAGAUAAAGGACCAA) and miRNA-mRNA EMSA revealed that miR-133a directly targets TIM17A (EMSA binding: WT 1.32±0.14 and mutant 0.40±0.22 using 3′UTR probes). In conclusion, we demonstrated that ...
miRNA's mimics (Gene Bank NO.: rno-miR-1, NR 032116.1; rno-mir-133a, NR 031879.1) and AMOs (AMO-1 and AMO-133a) were synthesized by Jima Inc (Shanghai, China). The sequences of miRNA mimics and AMOs are showed in Table 2. Table 2 The sequences of miRNA mimics and AMOs Full...
These miRNAs (miR145, miR-133a and miR-133b), which have conserved sequences in the 30UTR of FSCN1 (actin-binding protein, Fascin homolog 1), inhibited FSCN1 expression. The signal from a luciferase reporter assay was significantly decreased at 2 miR-145 target sites and 1 miR-133a/b...
The miRNA target sequences were inserted between theXhoI–PmeI restriction sites in the 3′UTR of thehRlucgene in the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). Primer sequences for full-length 3′UTR ofTAGLN2mRNA (5′-ATCGCTCGAGACAGATGGGCACCAACCGCG-3′ and 5′-CTCTAGGTTT...
随着 未来对基因簇 miR-1/ miR-133/ miR-206 调节机制的深入研究,将会开发出以 miRNA 为靶点的新型治疗手段,同时可能进一步促进心脏再生医学的发展。 参考文献 [1] AMERES SL, ZAMORE PD. Diversifying microRNA sequence and function [J]. Nat Rev Mol Cell Biol 2013; 14:475-488. [2] BARTEL DP. ...
We have recently identified down-regulated microRNAs including miR-145 and miR-133a in bladder cancer (BC). The aim of this study is to determine the genes targeted by miR-145, which is the most down-regulated microRNA in BC. We focused on fascin homolog
the expression of circRNA_100395 and KLHL20 mRNA in the myocardium of HF patients and healthy controls detected by RT-qPCR;D:identification of circRNA_100395 and KLHL20 gene by PCR assay;E:DNA sequencing results con...
47 No. 10 October 2024 表 1 qRT-PCR 实验使用的引物序列 Table 1 Primer sequences used for qRT-PCR 引物名称 miR-199a 正向 miR-199a 反向 Cel-miR-39-3p 正向 Cel-miR-39-3p 反向 序列(3'→5') GTCACAGTAGTCTGCACAT GTGCAGGGTCCGAGGT GGGTCACCGGGTGTAAATC GAGAGGAGAGGAAGAGGGAA 数和尺寸...
Each row represents a single sequence. The bar graphs depict the methylation rates of all 5 CpG sites (B) and single CpG sites (C). Full size image In order to further determine the causal role of DNA methylation in the enlargement of cell size and perturbations of key regulators of ...
Gain-of-function analysis revealed that 3 transfectants (miR-145, miR-133a and miR-133b) inhibit cell proliferation and cell invasion in ESCC cells. These miRNAs (miR-145, miR-133a and miR-133b), which have conserved sequences in the 3'UTR of FSCN1 (actin-binding protein, Fascin ...