In vertebrate spindles, most microtubules are formed via branching microtubule nucleation, whereby microtubules nucleate along the side of pre-existing microtubules. Hepatoma up-regulated protein (HURP) is a microtubule-associated protein that has been implicated in spindle assembly, but its mode of acti...
Microtubules are polarized cytoskeletal structures8, assembled from α-/β-tubulin heterodimers to form highly dynamic filaments, which play a crucial role in many physiological and pathological processes, including cell cycle and mobility9,10. Anti-tumor drugs targeting microtubules have recently been cu...
Here we show that a minimally reconstituted system composed of Klp2, a kinesin-14 from the fission yeast Schizosaccharomyces pombe, together with microtubules assembled from purified S. pombe tubulin, autonomously assembles bundles of parallel microtubules. Bundles form by an ATP-dependent sorting ...
1 and 2). Clathrin coats are assembled from two major components: clathrin and adaptor complexes (adaptor proteins; APs).1 APs are recruited from the cytosol onto the cytoplasmic surface of two distinct organelles: the plasma membrane and the trans-Golgi network (TGN). The binding of APs is...
Further understanding of the mechanism responsible for the uncoupling of the microtubule cytoskeleton from HIF-1α activity in RCC will lead to the development of more effective therapies for this disease. EXPERIMENTAL PROCEDURES Cell Lines PC3, MCF7, MDA-MB-231, ACHN, and CAKI-1 cells are ...
In vertebrate spindles, most microtubules are formed via branching microtubule nucleation, whereby microtubules nucleate along the side of pre-existing microtubules. Hepatoma up-regulated protein (HURP) is a microtubule-associated protein that has been i
Figures were assembled in Adobe Illustrator CS5 and Adobe Photoshop CS5 (Adobe Systems, Mountain View, CA, USA). Data are given as mean±s.e.m., unless otherwise stated. References 1. Cheeseman, I. M. & Desai, A. Molecular architecture of the kinetochore- microtubule interface. Nat. ...
Controlled swimming in confined fluids of magnetically actuated colloidal rotors. Phys. Rev. Lett. 101, 218304 (2008). Article Google Scholar Sing, C. E., Schmid, L., Schneider, M. F., Franke, T. & Alexander-Katz, A. Controlled surface-induced flows from the motion of self-assembled ...
Following preparation of a brain extract (HSS), MTs were cycled four times between an assembled polymeric state and a disassembled soluble state. P1–P4 are sequential MT pellets that were resolubilized prior to assay. Protein concentration in each fraction was measured, and total protein was from...
while the spindle aster assembled and moved into the decondensed chromatin regions. Kinetochores then moved towards the spindle pole, but the bulk mass of chromatin was not displaced towards the cell periphery such that the regions surrounding the spindle poles did not clear from chromatin, and the...