microRNA-99a心肌细胞细胞增殖目的有效地促进心肌细胞增殖为亟待发展的治疗策略。本研究拟观察高表达microRNA-99a(miR-99a)对体外培养心肌细胞增殖的影响并对其相关机制进行探讨。方法利用高表达miR-99a的慢病毒载体转染体外培养的乳鼠心肌细胞,诱导其在乳鼠心肌细胞中高表达。采用噻唑蓝法、EDU法检测乳鼠心肌细胞增殖能力...
产品简介:大鼠MicroRNA-99a检测试剂盒原理:用纯化的抗体包被微孔板,制成固相载体,往包被抗该指标抗体的微孔中依次加入标本或标准品、生物素化的抗该指标抗体、HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成Z终的黄色。颜色的深浅和样品中的该指标呈正相...
MicroRNA-99a/100 promotes apoptosis by targeting mTOR in human esophageal squamous cell carcinoma. Med Oncol. 2013;30(1):411.Jian Sun,Zhaoli Chen,Xiaogang Tan,Fang Zhou,Fengwei Tan,Yibo Gao,Nan Sun,Xiaohui Xu,Kang Shao,Jie He.  MicroRNA-99a/100 promotes apoptosis by targeting mTOR in ...
microRNA-99a结肠癌增殖迁移FGFR3目的:探讨结肠癌患者组织中microRNA-99a表达水平以及对结肠癌细胞增殖和迁移的影响. 方法: 取南京医科大学附属常州二院胃肠病中心49例结肠癌患者肿瘤组织,癌旁组织(癌旁5cm)标本以及结肠癌细胞HCT-116,HT-29,SW-480,Ca-co-2和正常结肠上皮细胞HCoePic,采用实时荧光定量PCR 检测结肠...
et al. MicroRNA-99a mimics inhibit M1 macrophage phenotype and adipose tissue inflammation by targeting TNFα. Cell Mol Immunol 16, 495–507 (2019). https://doi.org/10.1038/s41423-018-0038-7 Download citation Received28 November 2017 Revised21 April 2018 Accepted21 April 2018 Published30 May...
miR-99a还有增加neuro-2a细胞表达SNAP25的趋势。结论 miR-99a对过氧化氢诱导 neuro-2a细胞引起的氧化损伤具有保护作用。 【关键词】miR-99a;neuro-2a;过氧化氢;氧化损伤 【中图分类号】R743.9 EffectsofmicroRNA-99aonNeuro-2acellsagainstoxidativeinjuryinducedby hydrogenperoxide TaoZhen,WangRongliang,ZhaoHaiping,...
腺病毒介导microRNA-99a过表达抑制肝癌细胞生长 目的:利用腺病毒介导microrRNA-99a(miR-99a)的过表达,观察miR-99a对肝癌细胞生长的抑制作用,探讨一种腺病毒介导miRNA治疗肿瘤的新方法。方法:qRT-PCR检测正常肝细胞系... 张敬磊,金华君,刘辉,... - 《中国肿瘤生物治疗杂志》 被引量: 8发表: 2012年 ...
A diagnostic model was developed using a combined index of the levels of microRNA-99a and microRNA-125b in the urine supernatant with a sensitivity of 86.7%, a specificity of 81.1% and a positive predicted value (PPV) of 91.8%. Discriminating between high- and low-grade UCB, the model ...
比较两组患者疗效及 microRNA99a、miRNA145变化。结果 观察组治疗总有效率(783%)显著高于对照组(533%)(χ2=8336,P=0004);观察组治疗前 microRNA99a、miRNA145水平分别为(884±202)×10-6、(092±028),治疗后显著升高至(4223±1543)×10-6、(185±030)(P<005),对照组治疗前为(870±213)×10-6、(083...
Silencing circGFRA1 reduced p/t-AKT, p/t-FOXO1 and p/t-mTOR expression levels via upregulating miR-99a expression. In conclusion, our study demonstrated that knockdown circGFRA1 inhibits glioma cell proliferation and migration by upregulating microRNA-99a. 展开 ...