microRNA-9在脑组织中表达较为丰富,其在体内神经干或前体细胞分化发育中起着重要作用,但是microRNA-9对MSCs分化为神经细胞是否也存在促进作用目前尚无相关报道。Notch信号通路作为一条重要的信号通路可调控干细胞分化增殖,研究发现该通路参与MSCs分化为神经细胞这一进程。MSCs可以向神经细胞方向分化,但是microRNA-9是否...
研究微小RNA-9 (microRNA-9,miR-9)对胃癌SGC-7901细胞上皮-间充质转化(EMT)功能的影响及其相关机制.方法: SGC-7901胃癌细胞株分别转染miR-9 mimics和阴性对照序列(negative control mi-mic,NCM),作为miR-9组和NCM组,并设立未转染对照(control)组,采用RT-qPCR法检测各组细胞miR-9的含量,Transwell实验检测3组...
miR-9: a versatile regulator of neurogenesis Soon after its discovery, microRNA-9 (miR-9) attracted the attention of neurobiologists, since it is one of the most highly expressed microRNAs in the deve... C Marion,K Shauna,BC Laure - 《Frontiers in Cellular Neuroscience》 被引量: 144发表...
目的:探讨microRNA-9-1在体外诱导小鼠骨髓间充质干细胞(MSCs)向神经细胞分化中的作用.方法:构建小鼠microRNA-9-1慢病毒载体(microRNA-9-1-LV)并感染小鼠MSCs,筛选最适感染复数(MOI);实验分为未感染组、感染组(感染microRNA-9-1-LV)、阴性对照组(感染FU-RNAi-NC-LV);采用β-巯基乙醇诱导感染后小鼠MSCs分化...
长链非编码RNA AC138128.1靶向调控microRNA-9对胃癌细胞增殖及凋亡的影响 目的研究长链非编码RNA AC138128.1(lncRNA AC138128.1)对胃癌细胞增殖和凋亡的影响及microRNA-9(miR-9)在其中的作用.方法采用质粒转染的方式构建过表达lncRNA AC13812...
The functional significance of microRNA-9 (miR-9) during evolution is evidenced by its conservation at the nucleotide level from flies to humans but not its diverse expression patterns. Recent studies in several model systems reveal that miR-9 can regulate neurogenesis through its actions in neural...
我们发现miR-9(micro RNA-9,miR-9)被预测为靶向Fstl1的潜在miRNA之一.miR-9在心梗组织中表达下降,且与Fstl1表达呈负相关性.荧光素酶活性检测进一步证实miR-9靶向调控Fstl1.H9c2细胞中miR-9呈高表达的同时Fstl1表达下降,反之亦然.这表明miR-9靶向调控Fstl1且呈负相关性.为了进一步分析miR-9在缺氧细胞中的作用...
Set9, NF-κB, and microRNA-21 mediate berberine-induced apoptosis of human multiple myeloma cells. To investigate the mechanisms by which berberine suppressed the proliferation of human multiple myeloma cells.Human U266 multiple myeloma cell line was tes... Hai-yan,Hu,Kun-peng,... - 《Acta ...
A, sequences of the mir-9 microRNA and the 3′-UTR regions of rat oc2 gene recognized by mir-9. Underlined sequences correspond to the putative binding sites of mir-9 with the target gene. B, RT-PCR analysis of oc2, insulin (positive control), and β-actin (internal control) expre...
目的探讨microRNA-9(miR-9)参与调控卵巢癌细胞EMT过程,以及影响卵巢癌细胞侵袭及转移的分子机制.方法使用TargetScan及PicTar数据库,预测可能靶向E-cadherin 3'UTR区的miRNA,双荧光素酶报告体系进行验证;上调候选miR后,用qRT-PCR和Western blot检测E-cadherin的表达变化;细胞免疫荧光观察E-cadherin,?-Catenin和Vimentin的...