目的探讨microRNA-9(miR-9)参与调控卵巢癌细胞EMT过程,以及影响卵巢癌细胞侵袭及转移的分子机制.方法使用TargetScan及PicTar数据库,预测可能靶向E-cadherin 3'UTR区的miRNA,双荧光素酶报告体系进行验证;上调候选miR后,用qRT-PCR和Western blot检测E-cadherin的表达变化;细胞免疫荧光观察E-cadherin,?-Catenin和Vimentin的...
Set9, NF-κB and microRNA-21 mediate berberine-induced apoptosis of human multiple myeloma cells To investigate the mechanisms by which berberine suppressed the proliferation of human multiple myeloma cells.Human U266 multiple myeloma cell line was tes... Hai-yan,Hu,Kun-peng,... - 《Acta ...
MicroRNA-9 regulates survival of chondroblasts and cartilage integrity by targeting protogeninJinsoo Song, Dongkyun Kim, Churl-Hong Chun & Eun-Jung Jin Cell Communication and Signaling volume 11, Article number: 66 (2013) Cite this article ...
(3)microRNA可用于治疗肝脏疾病 由于microRNA在癌基因和肿瘤抑制因子中扮演着双重角色,科学家们开发了一些合成的 microRNA模拟物或拮抗物用于治疗癌症。例如,既往研究使用一种已失活的腺病毒将microRNA-22引入体内,从而抑制了肝癌的进展。与抗血管生成药物仑伐...
3 reagents referenced in The ROS-sensitive microRNA-9/9* controls the expression of mitochondrial tRNA-modifying enzymes and is involved in the molecular mechanism of MELAS syndrome.
Here, we found that microRNA-9 could promote MSC neuronal differentiation. Using immunocytochemistry, western blotting, and reverse transcription-PCR analyses, we showed that the expression of the neural cell specific marker, microtubule-associated protein 2, increased during the process, while the ...
The functional significance of microRNA-9 (miR-9) during evolution is evidenced by its conservation at the nucleotide level from flies to humans but not its diverse expression patterns. Recent studies in several model systems reveal that miR-9 can regulate neurogenesis through its actions in neural...
Sun et al. Molecular Neurodegeneration (2015) 10:36 DOI 10.1186/s13024-015-0032-9 RESEARCH ARTICLE Open Access MicroRNA-9 induces defective trafficking of Nav1.1 and Nav1.2 by targeting Navβ2 protein coding region in rat with chronic brain hypoperfusion Li-Hua Sun1*, Mei-Ling Yan1, Xue-...
Sun et al. Molecular Neurodegeneration (2015) 10:36 DOI 10.1186/s13024-015-0032-9 RESEARCH ARTICLE Open Access MicroRNA-9 induces defective trafficking of Nav1.1 and Nav1.2 by targeting Navβ2 protein coding region in rat with chronic brain hypoperfusion Li-Hua Sun1*, Mei-Ling Yan1, Xue-...
MicroRNA-9调节Navl.1和Navl.2膜转运的分子机制研究 图1钠通道基本结构图【24】 Figure 1The basic structure of sodium channel Navl.1在胞体和树突上以及郎飞氏结节点部位表达,Navl.2在成年人的 中枢神经系统中大量表达,特别是在皮层和海马区,主要定位在无髓鞘的轴突 以及聚集在突触起始端,维持动作电位时程的...