摘要: Adeno-associated virus (AAV)-mediated genetic targeting of microglia remains a challenge. Overcoming this hurdle is essential for gene editing in the central nervous system (CNS). Here, we characterized the minimal/native promoter of the关键词:...
and OPCs/oligodendrocytes in the white matter at a specific developmental stage is critical in supporting proper myelin synthesis. Besides, fractalkine-dependent microglial pruning of OPCs is indispensable for proper myelination. Mice lacking fractalkine receptor show a reduction in microglial phagocytosis ...
Depletion of microglia with CSF-1R inhibitor prevented motor deficits and neurodegeneration in mouse model of PD The lack of predictive power related to neuroprotection in acute neurotoxin-induced PD models has led to the development of progressive models of PD, e.g., the α-synuclein-induced PD ...
To visualize neuronal structures,Thy1-eGFPmice27were crossbred with 5xFAD transgenic mice co-expressing human APPK670N/M671L(Sw)+I716V(Fl)+V717I(Lo)and PS1M146L+L286Vunder the control of the neuron-specificThy1promoter28. To visualize green fluorescence in microglia, we intercrossedCx3cr...
Here we demonstrate that disruption of a conserved microglia-specific super-enhancer interacting with the Sall1 promoter results in complete and specific loss of Sall1 expression in microglia. By determining the genomic binding sites of SALL1 and leveraging Sall1 enhancer knockout mice, we provide ...
Microglia are the resident immune cells of the central nervous system (CNS), a tissue-resident macrophage population with specific characteristics to support the CNS environment and health. Microglia have a mesodermal origin and originate from yolk-sac progenitors during embryogenesis; after their early...
Future research should consider these factors in order to develop a comprehensive understanding of the immune response in males and females as these changes are likely the initiating agents that lead to the long-term, and often sex-specific, effects of early-life infection. 展开 关键词: ...
206 [45]. The molecular machinery that drives human microglia development and regional diversity remain unclear. Also, whether these clusters of microglia in selected brain regions reflect a region-specific function or susceptibility of microglia to human aging and AD remains to be elucidated....
Abbreviations 5-HT: 5-Hydroxy tryptamine ACC: Anterior cingulate cortex Ach: Acetylcholine ACTH: Adrenocor ticotropic hormone AG: Adrenal gland Akt1/Akt2: AKT serine/threonine kinase 1/2 Amy: Amygdala Arg1: Arginase-1 BBB: Blood-brain barrier ...
8a, right graphs) in microglia prepared from tamoxifen-inducible microglia-specific Pqbp1-cKO mice. Control plasmid expressed scrambled 20 nt gRNA. NFκB activity was monitored by two reporter plasmids expressing red fluorescent protein (RFP) or luciferase driven by NFκB promoter (Fig. 8b). NF...