Singh V, Mitra S, Sharma AK, Gera R, Ghosh D. Isolation and characterization of microglia from adult mouse brain: selected applications for ex vivo evaluation of immunotoxicological alterations following in vivo xenobiotic exposure. Chem Res Toxicol. 2014;27:895-903....
Mouse microglia isolation from mouse were isolated from adult mouse brain using the protocol as described before66. Briefly, microglia-enriched percoll fractions were sorted by gating the DAPInegCD11bhighCD45intLy6cneg cells. 200.000-300.000 microglia per brain were sorted for RNAseq experiments. ...
Although genetics highlights the role of microglia in Alzheimer’s disease, one-third of putative Alzheimer’s disease risk genes lack adequate mouse orthologs. Here we successfully engraft human microglia derived from embryonic stem cells in the mouse brain. The cells recapitulate transcriptionally huma...
followed by FACS isolation of carboxyfluorescein succinimidyl ester (CFSE)-labelled replenished and CFSE-endogenous microglia that differentially phagocytose endogenous NIAD4-labelled plaques after 5 days co-culture with wild-type (WT)
Microglia isolation from adult mouse brain. Methods Mol Biol. 2013;1041:17–23. Article PubMed PubMed Central CAS Google Scholar Hohsfield LA, Humpel C. Migration of blood cells to beta-amyloid plaques in Alzheimer’s disease. Exp Gerontol. 2015;65:8–15. Article PubMed PubMed Central ...
Isolation of region-specific microglia from one adult mouse brain hemisphere for deep single-cell RNA sequencing. J Vis Exp. 2019;3(154):e60347.10.3791/60347Search in Google Scholar PubMed [33] Nikodemova M, Watters JJ. Efficient isolation of live microglia with preserved phenotypes from ...
A method has been developed to isolate microglia from adult human and rat brain cell suspensions by rosette formation via Fc receptors. Immunocytochemical characterisation of the cells immediately following isolation and after 7-10 days in vitro with a panel of monoclonal antibodies has demonstrated tha...
Isolation of immune cells from mouse brain Mice were housed, bred and handled according to the Institutional Animal Care and Use Committee guidelines. Brains were isolated from brain cortices; meninges and choroid plexi were removed. Remaining brain tissue was mechanically dissociated in Dulbecco's pho...
aSchematic representing the experimental design involving addition of 2 × 104microglia to NIAD4-stained organotypic hippocampal slice cultures (OHSCs), followed by FACS isolation of carboxyfluorescein succinimidyl ester (CFSE)-labelled replenished and CFSE-endogenous microglia that differentially phagocy...
Microglia isolation from mice and flow cytometry After sham or tMCAO surgery, mice were killed by cervical dislocation at certain time points. The right hemisphere of the tMCAO brains or sham brains, excluding brain stem and olfactory bulbs, were cut into pieces with tissue scissors and then pu...