cultureFACSfluorescence‐activated cell sortingimmunopanningMACSmagnetic‐activated cell sortingmicrogliatissue‐resident macrophageMicroglia represent 5‐10% of cells in the central nervous system and contribute to the development, homeostasis, injury, and repair of neural tissues. As the tissue‐resident ...
Isolation and culture of primary human nasal epithelial cells from anesthetized nasal epithelia Microglia, the resident immune cells of the brain, have been implicated in numerous neurodegenerative and neurodevelopmental diseases. Activation of microg... R Hussain,S Hugosson,GM Roomans 被引量: 0发表...
Tags Isolation Culture Mouse Cortical Astrocytes Glial Cells In Vitro Cell Culture Systems Biological Functions Mixed Cortical Cells Central Nervous System Pure Astrocytes Microglia Oligodendrocytes Immunocytochemical Stainings Astrocyte Markers Astrocyte Biology Article...
Microglia and astrocytes are implicated in aging and age-related diseases. Here, we present a protocol to isolate and culture these glia cells from the mur... MT Milner,GM Lawrence,CL Holley,... - 《Star Protocols》 被引量: 0发表: 2022年 Isolation, Culture and Transduction of Adult Mouse...
We describe a technique to isolate two major phenotypically different populations of microglia (type I and type II) in mixed brain culture. Type I microglia have surface markers for mature monocytic cells and produce microglia-specific cytokines. Type II microglia have cell-surface characteristics excl...
Tumor tissue storage and dissociation Tumor cell isolation Tumor cell culture Tumor infiltrating leukocytes Circulating tumor cells Tumor microenvironment Exosome isolation and analysis Neural cells overview Neuronal cells Astrocytes Oligodendrocytes Microglia Adult brain cells Flow cytometry applic...
In the study of Dick et al., the retinas were disrupted mechanically through a stainless steel sieve to isolate the microglia, and flow cytometry analysis revealed that the proportion of microglia was up to 8% [25]. We speculate that: (1) In in vitro culture, microglia grow semi-adherent...
175cm² Angled Neck Cell Culture Flask with Vent Cap Corning 431080 Lipopolysaccharides from Escherichia coli O127:B8 Sigma-Aldrich L5024 96 Well TC-Treated Microplates size 96 wells, clear, polystyrene, round bottom Corning CLS3799 Paraformaldehyde (powder, 95%) Sigma-Aldrich 158127...
A new method to isolate microglia from adult mice and culture them for an Extended period of time As the major immuno-competent cells of the brain, microglia are highly implicated in neuro-protection as well as in neurodegeneration. Therefore, they are ... S Moussaud,HJ Draheim - 《Journal...
Cerebral microvessels and derived cells in tissue culture: Isolation and preliminary characterization Microvessels isolated from mouse forebrain were used as the source material for the derivation of cerebral vascular endothelium and smooth-muscle cells in ... LE Debault,LE Kahn,FPA Cancilla - In Vi...