Human MHC-I consists of an a-chain encoded by HLA genes (HLA-A, HLA-B and HLA-C) and a noncovalently associated b2m subunit. We used a variety of techniques to assess the presence of MHC-I in human postmortem samples. First, we examined MHC-I expression by immunofluorescence using a ...
The mRNA expression of the genes involved in MHC class I antigen presentation and processing, including B2M, LMP2 and TAP1, was also reduced in the NLRC5-defi- cient spleen (Figure 1A) and thymus (Supplementary in- formation, Figure S2A), whereas the mRNA expression of MHC class II ...
MHC-I: Major histocompatibility complex class I HLA-ABC: Human Leukocyte Antigen Class 1 ABC B2M: Beta-2 microglobulin RT-PCR: Quantitative reverse transcription polymerase chain reaction MFI: Median fluorescence intensity FCM: Flow cytometry Az: Azelastine H&E: Hematoxylin and eosin IHC...
Common mechanisms of evasion include impaired antigen presentation caused by mutations or loss of heterozygosity of the major histocompatibility complex class I (MHC-I), which has been implicated in resistance to immune checkpoint blockade (ICB) therapy1,2,3. However, in pancreatic ductal ...