The relation betweenmfeprimerand its sub-commands:mfeprimerdo a full quality control for primers for specificity, binding sites, dimers and hairpins. While,speconly for specificity,dimeris only for dimers andhairpinis only for hairpins, as their names indicated. The sub-commands have more options...
Mfeprimer下载:https://github.com/quwubin/MFEprimer-3.0/releases 本人最近在筛选分子标记,然后发现合成了很多引物,但是多态性并不是很好,然后查阅了相关资料后发现一款软件mfeprimer能对我们的引物进行自由组合并得到预选的扩增条带,所以这里记录一下。当然这也叫电子pcr了哈。 安装教程官网也给出了:https://www.mfe...
MFEprimer Web Server 4.0 # The MFEprimer Web Server version 4.0 serves as a comprehensive platform for PCR primer quality control and design tools. We have recently launched a new feature, the MP2, which is a multiplex PCR primer design tool. Additionall
Primer Opt Tm Primer Max Tm Product Min Size Product Max Size MFEprimer Parameters Dimer Score Cutoff Hairpin Score Cutoff Tm Min °C Minimum Tm value required for the binding stability between primer and its binding sites. Nonspecific Amplicon Size (bp) ...
linux-amd64# test it./mfeprimer-3.1.0-linux-amd64 -hMFEprimer-3.1.0, a functional primer quality control program for checkingnon-specific amplicons, dimers, hairpins and others.Web site: https://www.mfeprimer.com/Cite: Wang, K., Li, H., Xu, Y., Shao, Q., Yi, J., Wang, R.,...
【3.2】MFEprimer 一、简介 功能介绍: dimer analysis primer dimers hairpin analysis primer hairpins help Help about any command index index fasta file for mfeprimer indexcmp Compare the old and the new index file, return false when they are not same...
软件名称基于MFEprimer3的引物设计评估WEB系统 软件简称MFEprimer引物设计评估WEB系统版本号1.0 登记号2024SR2184923分类号- 著作权人中国人民解放军军事科学院军事医学研究院首次发表日期- 登记日期2024-12-25 该公司其他软件著作权 序号登记日期软件全称软件简称登记号版本号 ...
网络多因素引物特异性评估系统 网络释义 1. 多因素引物特异性评估系统 第2章 基于多因素引物特异性评估系统(MFEprimer)构建第26-34页 ·主要参数的添加与修订 第26-29页 ·解链温度Tm计算 第26-2… www.lunwentianxia.com|基于4个网页
PCR Primer Design MFEprimer for Multiplex PCRis a product designed specially for multiplex PCR application, usually for large number of SNPs genotyping, following with NGS. Workflow Reference: Wang, K., et al. (2019). "MFEprimer-3.0: quality control for PCR primers." Nucleic Acids Research....
MFEprimer 来自 ACM 喜欢 0 阅读量: 373 作者:W Qu,Z Shen,D Zhao,Y Yi,C Zhang 摘要: Summary: We developed a program named MFEprimer for evaluating the specificity of PCR primers based on multiple factors, including sequence similarity, stability at the 3 ′ -end of the primer, melt......