Mfeprimer下载:https://github.com/quwubin/MFEprimer-3.0/releases 本人最近在筛选分子标记,然后发现合成了很多引物,但是多态性并不是很好,然后查阅了相关资料后发现一款软件mfeprimer能对我们的引物进行自由组合并得到预选的扩增条带,所以这里记录一下。当然这也叫电子pcr了哈。 安装教程官网也给出了:https://www.mfe...
test it. # download the executable mfeprimer-web filewget -c https://github.com/quwubin/MFEprimer-3.0/releases/download/v3.1.1/mfeprimer-web-3.1.1-darwin-amd64.tar.gz# uncompress the filetar zxvf mfeprimer-web-3.1.1-darwin-amd64.tar.gz# The file tree is:tree -L2.├── conf│ └─...
Download and Online Servers Online servers# [v4.0]https://m4.igenetech.com/ [v3.1]https://mfeprimer3.igenetech.com/ Download# MFEprimer all releases:https://github.com/quwubin/MFEprimer-3.0/releases/ MFEprimer command-line usage.
test it. # download the executable mfeprimer-web filewget -c https://github.com/quwubin/MFEprimer-3.0/releases/download/v3.1.1/mfeprimer-web-3.1.1-darwin-amd64.tar.gz# uncompress the filetar zxvf mfeprimer-web-3.1.1-darwin-amd64.tar.gz# The file tree is:tree -L2. ├── conf │ ...
1.1 安装 centos7上的安装 wget -c https://github.com/quwubin/MFEprimer-3.0/releases/download/v3.2.2/mfeprimer-3.2.2-linux-amd64.gz gzip -d mfeprimer-3.1.0-linux-amd64.gz chmod +x mfeprimer-3.1.0-linux-amd64 # test it ./mfeprimer-3.1.0-linux-amd64 -h ...
# download the executable mfeprimer filewget -c https://github.com/quwubin/MFEprimer-3.0/releases/download/v3.1.0/mfeprimer-3.1.0-linux-amd64.gz# uncompress the filegzip -d mfeprimer-3.1.0-linux-amd64.gz# make it executablechmod +x mfeprimer-3.1.0-linux-amd64# test it./mfeprimer-3.1.0...