Dimer Check: Assess your primers for potential dimer formations, which can inhibit PCR performance.Try it here. Hairpin Check: Evaluate primers for hairpin structures that can negatively impact PCR efficiency.Try it here. Coverage Analysis for Microbes: This new addition assesses how effectively a ...
Product Min Size Product Max Size MFEprimer Parameters Dimer Score Cutoff Hairpin Score Cutoff Tm Min °C Minimum Tm value required for the binding stability between primer and its binding sites. Nonspecific Amplicon Size (bp) From To Product size: 0 (min) - 1000000 (max)....
The relation betweenmfeprimerand its sub-commands:mfeprimerdo a full quality control for primers for specificity, binding sites, dimers and hairpins. While,speconly for specificity,dimeris only for dimers andhairpinis only for hairpins, as their names indicated. The sub-commands have more options...
gzip -d mfeprimer-3.1.0-linux-amd64.gz chmod +x mfeprimer-3.1.0-linux-amd64 # test it ./mfeprimer-3.1.0-linux-amd64 -h 二、用法示例 2.1 多条引物dimmer的检验 /data/software/mfeprimer-3.2.2-linux-amd64 dimer -i fasta/test.fa -o tmp2.tsv -j -s 4 --primer 参数说明: -i 输入...
Nucleic Acids Research.https://doi.org/10.1093/nar/gkz351.Usage: mfeprimer [flags] mfeprimer [command]Available Commands: dimer analysis primer dimers hairpin analysis primer hairpins help Help about any command index index fasta file for mfeprimer indexcmp Compare the old and the new index file...
Primer Opt Size Primer Max Size Primer Min Tm Primer Opt Tm Primer Max Tm Product Min Size Product Max Size MFEprimer Parameters Dimer Score Cutoff Hairpin Score Cutoff Tm Min °C Minimum Tm value required for the binding stability between primer and its binding sites. ...
Runmfeprimer dimerfor dimers# ./mfeprimer dimer -i p.fa Runmfeprimer hairpinfor hairpins# ./mfeprimer hairpin -i p.fa 3. Local web server# Download and install# Please download the right version for your machine:https://github.com/quwubin/MFEprimer-3.0/releases(take the Mac version as an...