(kcal/mol) Plus Minusp1 CTACAACCCCACCACGTACC 20 60.00 60.55 -22.23 0 0p2 CGTTACACACTTTGCGGCAA 20 50.00 60.47 -22.43 0 0p3 CTTAAATAGGGACCTGTATGAATGGCTC 28 42.86 61.98 -27.11 2 1p4 CCGAAATTTTTAATGCAGGTTTGGTAGT 28 35.71 61.97 -27.14 0 1p5 GGACACTCTATGGGAAAGAGTGTCC 25 52.00 62.91 -...
摘要: Summary: We developed a program named MFEprimer for evaluating the specificity of PCR primers based on multiple factors, including sequence similarity, stability at the 3 ′ -end of the primer, melt...被引量: 61 年份: 2009 收藏 引用 批量引用 报错 分享 ...
# download the executable mfeprimer filewget -c https://github.com/quwubin/MFEprimer-3.0/releases/download/v3.1.0/mfeprimer-3.1.0-linux-amd64.gz# uncompress the filegzip -d mfeprimer-3.1.0-linux-amd64.gz# make it executablechmod +x mfeprimer-3.1.0-linux-amd64# test it./mfeprimer-3.1.0...