To identify the altered metabolism-related pathways and hub genes in sPTB patients, we performed multiple functional enrichment analysis and applied three machine learning algorithms, LASSO, SVM-RFE, and RF, with the hub genes that were verified by immunohistochemistry. Additiona...
We performed gene set enrichment analysis (GSEA) using Hallmark Gene Sets from the Molecular Signatures Database (MSigDB) to identify different functional pathways within the molecular subtypes [22]. Evaluation of the tumor microenvironment and responses to immunotherapy The CIBERSORT algorithm (https:/...
In addition, GSEA identified various pathways commonly associated with three key DELMRGs. Moreover, the regulatory network of miRNA-TF for key DELMRGs was established. Finally, significant differences in immune cell levels and several pathways were identified between the two subclusters. Conclusion ...
In the human body, vitamin B6 is primarily absorbed in the small intestine, where it enters the portal vein and subsequently reaches the liver, then it is converted into PL, PN and PM as well as their respective 5'-phosphates [4]. A portion of pyridoxine 5'-phosphate (PNP) and pyridoxa...
Background: Osteosarcoma is one of the most common malignant neoplasm among children and adolescents. Studies have shown that metabolism-related pathways are more important for the development and metastasis of osteosarcoma. Long non-coding RNA (LncRNA) plays a key role in the occurrence and progres...
Here, we present a protocol for analysing incorporation of the non-radioactive stable isotopes carbon-13 (13C) and nitrogen-15 (15N) into polar metabolites in central carbon metabolism and related pathways. Our platform utilizes selected reaction monitoring (SRM) with polarity switching and amide ...
We also investigated gene interaction with miRNAs and immune infiltration analysis.#Through these steps, we provided a list of possible diagnostic markers and therapeutic targets for ccRCC.#The current study further proved the importance of mitochondrial metabolic pathways in the pathogenesis of ccRCC ...
Three pathways of net carbon assimilation and reduction have been detected in certain higher plant. The three recognized pathways are: the reductive pentose phosphate, the C4-dicarboxylic, and crassulacean acid metabolism. Leaves of a given plant may exhibit at least one of these pathways. A plant...
In contrast, the high-risk patients were more associated with non-metabolic pathways. In summary, our study provides novel insight into metabolism-related genes’ roles in ccRCC.Similar content being viewed by others Better prediction of clinical outcome in clear cell renal cell carcinoma based on ...
pathways upregulated in the C1 subtype than in the C3 subtype, except hypoxia.FThe top 3 HALLMARK signaling pathways upregulated in the C3 subtype than in the C1 subtype, except hypoxia. DEHRGs: different expressed hypoxia-related genes; DELMRGs: different expressed lactate metabolism-related ...