Post-translational modification of proteins with ubiquitin (ubiquitination) provides a rapid and versatile mechanism for regulating cellular signalling systems. Met1-linked (or 'linear') ubiquitin chains have emerged as a key regulatory signal that controls cell death, immune signalling, and other vital...
Here, we show that ABIN-1/NAF-1, a ubiquitin-binding protein, is recruited rapidly into TNF-RSC in a manner dependent on the Met1-ubiquitylating complex LUBAC to regulate the recruitment of A20 to control Lys63 deubiquitylation of RIPK1. ABIN-1 deficiency reduces the recruitment of A20 ...
The majority of these mutations hinder the ability of pVHL, a component of the E3 ubiquitin ligase complex ECV, to target hypoxia inducible factor a (HIFa) for polyubiquitinated degradation (Ohh et al., 1999). HIF1a is a transcription factor that activates a number of genes involved in ...
Mitochondria are highly dynamic organelles that are continually undergoing fission and fusion.20Altered mitochondrial dynamics have been linked to abnormal cell functions21,22and many human diseases,23,24,25,26including cancers.26,27Studies have shown that excess mitochondrial fission28,29and upregulation...
39. The vector of claim 38, wherein said vector further comprises a regulatory sequence, which is operably linked to said nucleic acid sequence. 40. The vector of claim 39, wherein said vector is an expression vector. 41. A host transformed or transfected with a vector defined in claim ...
N-terminal methionine-linked ubiquitin (Met1-Ub), or linear ubiquitin, has emerged as a central post-translational modification in innate immune signalling. The molecular machinery that assembles, senses and, more recently, disassembles Met1-Ub has been identified, and technical advances have ...
Summary Conjugation of Met1-linked polyubiquitin (Met1-Ub) by the linear ubiquitin chain assembly complex (LUBAC) is an important regulatory modification in innate immune signaling. So far, only few Met1-Ub substrates have been described, and the regulatory mechanisms have remained elusive. We ...
The enzyme-mediated construction of poly ubiquitin (Ub) chains on target proteins leads to a variety of cellular responses and is involved in processes ranging from protein degradation to cell cycle control and immune responses. This complex post-translational modification system is under intense ...
Summary The linear ubiquitin (Ub) chain assembly complex (LUBAC) is an \\{E3\\} ligase that specifically assembles Met1-linked (also known as linear) Ub chains that regulate nuclear factor κB (NF-κB) signaling. Deubiquitinases (DUBs) are key regulators of Ub signaling, but a dedicated...
We have reported previously that activation of the MyD88-signaling network rapidly induces the formation of hybrid ubiquitin chains containing both Lys63-linked and Men-linked ubiquitin (Ub) oligomers, some of which are attached covalently to Interleukin Receptor Associated kinase 1. Here we show ...