However, the mechanism by which NO effects covalent attachment of nucleotide and its role in regulation of enzyme activity are controversial. Recent studies ... S Mohr,JS Stamler,B Brüne - 《Journal of Biological Chemistry》 被引量: 483发表: 1996年 Carbonic anhydrase inhibitors: inhibition of ...
The results show that these N-substituted morpholines strongly inhibit this enzyme (I50: 0.4 μM for fenpropimorph, 0.6 μM for tridemorph). In fenpropimorph, where a chiral centre is present at C-2 of the alkyl chain, good enantioselectivity was observed for enzyme inhibition. Inhibition of ...
et al. Autophagy inhibition suppresses pulmonary metastasis of HCC in mice via impairing anoikis resistance and colonization of HCC cells. Autophagy 9, 2056–2068 (2013). Article PubMed CAS Google Scholar Cai, Q., Yan, L. & Xu, Y. Anoikis resistance is a critical feature of highly ...
2c). The A2 loop of bsIRG1 was localized away from the active site, while the A2 loop of IDS epimerase was close to the active site. This indicates that the A2 loop might be flexible in the MmgE/PreD protein family and control the open and closed conformations of this enzyme family ...
Kinetic analysis of the formation of the 75 kDa iPLA2β-BEL species with the (R) and (S) enantiomers of BEL demonstrated that the reaction of (S)-BEL with iPLA2β was more rapid than for (R)-BEL paralleling the enantioselectivity for the inhibition of catalysis by each inhibitor with ...
Fluorometric reading of enzyme control (Con) was defined as 100% COX activity. COX-1 specific inhibitor SC560 (SC) and COX-2 specific inhibitor celecoxib (Cel) served as controls. *P < 0.05 (ANOVA); n = 7 Full size image YXT and its herbal components exert multi-target effects...
The main inhibition mechanism of kaempferol on XO activity may be due to the insertion of kaempferol into the active site of XO occupying the catalytic center of the enzyme to avoid the entrance of the substrate and inducing conformational changes of XO. In addition, luteolin exhibited a ...
Electric eel enzyme revealed the highest inhibition extent with a binding constant estimated to 0.35 mu M. This binding prevents the entrance of substrate en route to the catalytic site and also decreases chemical steps of the reaction at the catalytic site: acetylation is reduced to the half ...
Ornithine decarboxylase from Lactobacillus 30a is gradually inactivated by treatment with alpha-methylornithine, but activity is restored by treatment of the inactivated enzyme with pyridoxal phosphate. Inactivation of the enzyme is associated with formation of pyridoxamine phosphate and 5-amino-2-pentanone...
To avoid the confounding effects of two replication forks converging on a circular template, we first performed reactions on linear templates. Plasmids were linearised with a restriction enzyme (AhdI) such that the replication origin was positioned 1.5 kb from one end, and 8.2 kb from the...