DNA ligase III contains a unique N-terminal zinc finger domain that binds to nicks and gaps in DNA. This small domain has been described as a nick sensor, but it is not required for DNA nick joining activity in vitro. Recent structural studies of other human DNA ligases identified other ...
Examining the nature of 9-1-1 interaction with DNA ligase I, we show that there is a similar degree of stimulation on ligation substrates with different structures, and that there is specificity for DNA ligase I. 9-1-1 improves the binding of DNA ligase I to nicked double strand DNA. ...
The A-tailing products (100 ng) were ligated with a dsDNA adapter containing a 3’-dT overhang (100 ng) at 16 °C for 1 h using 1 μL T4 DNA ligase (TAKARA) in 20 μL reaction volume. After ligation, the adapter-bearing cleavage products were PCR amplified to obtain ...
Pol μ is capable of performing gap-filling repair synthesis in the nonhomologous end joining (NHEJ) pathway. Together with DNA ligase, misincorporation of dGTP opposite the templating T by Pol μ results in a promutagenic T:G mispair, leading to genomic instability. Here, crystal structures an...
Accurate chromosomal DNA replication is essential to maintain genomic stability. Genetic evidence suggests that certain repetitive sequences impair replication, yet the underlying mechanism is poorly defined. Replication could be directly inhibited by th
What is an alteration in the DNA structure or sequence of a gene called? Which enzyme is responsible for copying DNA into an RNA transcript? a. helicase b. spliceosome c. DNA polymerase d. RNA polymerase e. ligase a. What are the major enzymes or mechanisms needed for the ...
Loss of SPRTN results in failure to repair DPCs and hypersensitivity to DPC-inducing agents. SPRTN accomplishes DPC processing through a unique DNA-induced protease activity, which is controlled by several sophisticated regulatory mechanisms. Cellular, biochemical, and structural studies define a DNA ...
of DNA methylation in RA development, bridging the gap between genetic variations and patient outcomes [31]. Abnormal hyperplasia of RA synovial fibroblasts (RASFs) plays a pivotal role in driving the progression of RA and subsequent joint destruction. Takami et al. identified the hypermethylation ...
AMP-dependent DNA relaxation catalyzed by DNA ligase occurs by a nicking-closing mechanism. In the presence of AMP and , a covalently closed duplex DNA containing negative superhelical turns was treated with isolated from -infected . This resulted... M Alessandra,C Giovanni - 《Nucleic Acids Res...
Prokaryotic and eukaryotic expression constructs were generated by standard cloning techniques, using Phusion polymerase, restriction digestion, and ligation by T4 DNA ligase. Point mutants were generated by quick-change site-directed mutagenesis, using Phusion polymerase. A detailed overview of all expressi...