Mitochondrial DNA ligase function in 来自 国家科技图书文献中心 喜欢 0 阅读量: 24 作者: Donahue,L S.摘要: The Saccharomyces cerevisiae CDC9 gene encodes a DNA ligase protein that is targeted to both the nucleus and the mitochondria. While nuclear Cdc9p is known to play an essential role in...
Many DNA ligases also contain a non-catalytic domain that is conserved, but the function of this domain is unknown. ATP-dependent ligases (based on Bacteriophage T7) It is two domain ligase, the first one is the adenylation or nucleotide-binding domain. This domain serves site for ATP bindin...
The function of DNA ligase in genetic engineering is to stick cut pieces of DNA together. Inside cells, DNA ligase is an enzyme necessary for DNA...Become a member and unlock all Study Answers Start today. Try it now Create an account Ask a question Our experts can answer your tou...
DNA ligase is an enzyme that forms a covalent phosphodiester bond between a 3′ OH and 5′ PO4 at the ends of two polynucleotide chains (frequently at a nick in one strand of a double helix). From: DNA Structure and Function, 1994 ...
T4 phage completely defective in both gene 30 (DNA ligase) and the rII gene (function unknown) require at least normal levels of host-derived DNA ligase (E. coli lig gene) for growth. Viable E. coli mutant strains that harbor less than 5% of the wild-type level of bacterial ligase do...
(1998) DNA ligase I selectively affects DNA synthesis by DNA polymerases delta and epsilon suggesting differential functions in DNA replication and repair. J. Biol. Chem. 273, 14322-14330Mossi, R., Ferrari, E. and Hubscher, U. (1998) DNA ligase I selectively affects DNA synthesis by DNA ...
We identify yeastlike tRNA ligases in the proteomes of Leishmania and Trypanosoma. These findings recommend tRNA ligase as a target for antifungal and antiprotozoal drug discovery.Wang LKShuman SRNAWang, L.K. and Shuman, S. 2005. Structure-function analysis of yeast tRNA ligase. RNA 11: 966...
To better understand the unique features of LigIV function, here we report the crystal structure of the catalytic core of human LigIV in complex with a nicked nucleic acid substrate in two distinct states—an open lysyl-AMP intermediate, and a closed DNA–adenylate form. Results from structural...
et al. A Dominant Mutation in Human RAD51 Reveals Its Function in DNA Interstrand Crosslink Repair Independent of Homologous Recombination. Mol. Cell 59, 478–490 (2015). Article CAS PubMed PubMed Central Google Scholar Auerbach, A. D. Fanconi anemia and its diagnosis. Mutat. Res. - ...
A computational approach for identifying functionally relevant SNPs in gene LIG1 has been proposed. LIG1 is a crucial gene which is involved in excision repair pathways and mutations in this gene may lead to increase sensitivity towards DNA damaging agents. A total of 792 SNPs were reported to ...