As a consequence of multiple protein interactions, ligases activity may be enhanced to complete a specific strand joining reaction. In humans, three ligase genes encoding four ligases (ligase I, ligase IIIalpha, ligase IIbeta, and ligase IV) are required for replication, recombination, repair, ...
Accurate chromosomal DNA replication is essential to maintain genomic stability. Genetic evidence suggests that certain repetitive sequences impair replication, yet the underlying mechanism is poorly defined. Replication could be directly inhibited by th
The mixture (50 μl) in annealing buffer (50 mm Tris·HCl, pH 7.4, 100 mm NaCl) was heated to 65 °C for 10 min, cooled to 16 °C, and then adjusted to 10 mm MgCl2 plus 2 mm ATP, and 50 units of T4 DNA ligase was added. Ligation was for 10 h at 16 °C. Then the ...
CRISPR-Cas systems and IS200/IS605 transposon-associated TnpBs have been utilized for the development of genome editing technologies. Using bioinformatics analysis and biochemical experiments, here we present a new family of RNA-guided DNA endonucleases.
The reaction was diluted into 4 ml ligation buffer containing 50 U of T4 DNA Ligase and incubated at 16 °C for 18 h. Samples were de-crosslinked by incubation at 65 °C in the presence of proteinase K for 15 min, purified by phenol/chloroform extraction and ethanol ...
Inhibition of ATP binding by drugs, such as geldanamycin, blocks client protein activation. dimer to relax to a default state (Figure 5). The currently accepted model for that tense ATP-bound state of the Hsp90 dimer is sim- ilar to the comparable state of the DNA gy- rase B and MutL...
This recruitment is dependent on the presence of both HR1 and HR2 and suggests they may interact with each other [30]. Additionally, the C-terminus of Mfn2, lacking the transmembrane region can be immunoprecipitated with the N-terminus of Mfn2 in a reaction which depends on the GTPase ...
Cotton boll shedding is one of the main factors adversely affecting the cotton yield. During the cotton plant growth period, low light conditions can cause cotton bolls to fall off prematurely. In this study, we clarified the regulatory effects of low li
Silberg J. J., Tapley T. L., Hoff K. G. and Vickery L. E. (2004) Regulation of the HscA ATPase reaction cycle by the cochaperone HscB and the iron-sulfur cluster assembly protein IscU. J. Biol. Chem.279: 53924–53931 ArticlePubMedCASGoogle Scholar ...
As swapping proceeds, new protein and DNA species become further separated from each other, as protein oligomers elute earlier than the parent monomer and digested DNA products elute later than the parent oligonucleotide. Figure 4: FK506 induces dimerization of SF70 and activates enzymatic activity....