As such, RtcB is a core factor in protein synthesis and homeostasis. Taking advantage of the high homology between RtcB orthologues in archaea, bacteria and eukaryotes, this review will provide an introduction to the structure of RtcB and the mechanism of 3′–5′ RNA ligation. This analysis ...
K. Structure-Function Correlations Derived from Faster Variants of a RNA Ligase Deoxyribozyme. ... Tracey K Prior,Daniel R Semlow,Amber Flynn-Charlebois,... 被引量: 2发表: 2018年 A DNA metalloenzyme with DNA ligase activity retain the ability to cleave RNA, and a 'deoxyribozyme' was ...
duplexed DNA target. These structures explain the mechanism ofprotospacer adjacent motif(PAM) recognition and reveal an elongation of the complex that is driven by crRNA-guided hybridization to cDNA. However, the mechanism by which the nuclease is recruited to the target-bound complex was not ...
The results show how Elongin binds to Pol II, reveal the exact regions required for the elongation-stimulatory function of Elongin and elucidate the mechanism of elongation stimulation by Elongin. Results Stimulation of Pol II elongation by recombinant human Elongin We prepared human Elongin (Fig. ...
Therapeutic mRNAs and vaccines are being developed for a broad range of human diseases, including COVID-19. However, their optimization is hindered by mRNA instability and inefficient protein expression. Here, we describe design principles that overcome these barriers. We develop an RNA sequencing-bas...
select article Molecular Insights into Hydrogen Peroxide-sensing Mechanism of the Metalloregulator MntR in Controlling Bacterial Resistance to Oxidative Stresses Research articleOpen access Molecular Insights into Hydrogen Peroxide-sensing Mechanism of the Metalloregulator MntR in Controlling Bacterial Resistance ...
Mechanism of action of a yeast RNA ligase in tRNA splicing - ScienceDirect The yeast endonuclease and ligase activities that carry out the splicing of tRNA precursors in vitro have been physically separated. The properties of a pa... Chris,L.,Greer,... - 《Cell》 被引量: 377发表: 1983年...
Structure-function analysis of Methanobacterium thermoautotrophicum RNA ligase--engineering a thermostable ATP independent enzyme.Structure-function analysis of Methanobacterium thermoautotrophicum RNA ligase--engineering a thermostable ATP independent enzyme.Background: RNA ligases are essential reagents for many...
Crystal Structures of Human DNA Polymerase β Complexed with Gapped and Nicked DNA: Evidence for an Induced Fit Mechanism , DNA polymerase beta (pol beta) fills single nucleotide (nt) gaps in DNA produced by the base excision repair pathway of mammalian cells. Crystal structures... MR Sawaya,...
structure models including the 3′ fragment of RNU7 (a small nuclear RNA, snRNA, AUC = 0.994) and Gln-TTG-2-1 (a tRNA, AUC = 0.818) (Supplementary Fig.1d). Furthermore, when comparing the structure scores to the secondary structure models of tRNAs from GtRNAdb19, the single-...