FIGURE 1.16.Schematic mechanism of nuclear mRNA splicing. The intron splicing of a pre-mRNA occurs in a spliceosome, a complex structure in which at least five snRNPs recognize, associate with the pre-mRNA, and assist in the correct 5′ and 3′ cleavages of the intron. The recognition of ...
As such, RtcB is a core factor in protein synthesis and homeostasis. Taking advantage of the high homology between RtcB orthologues in archaea, bacteria and eukaryotes, this review will provide an introduction to the structure of RtcB and the mechanism of 3′–5′ RNA ligation. This analysis ...
Synthesis of spin-labeled riboswitch RNAs using convertible nucleosides and DNA-catalyzed RNA ligation. Bioorg. Med. Chem. 21, 6171–6180 (2013) Article PubMed CAS Google Scholar Vicens, Q. & Cech, T. R. A natural ribozyme with 3′,5′ RNA ligase activity. Nature Chem. Biol. 5, 97...
Structure and mechanism of RNA polymerase II CTD phosphatases Mol. Cell, 15 (2004), pp. 399-407 View PDFView articleView in ScopusGoogle Scholar Kimura et al., 2002 M. Kimura, H. Suzuki, A. Ishihama Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol...
The results show how Elongin binds to Pol II, reveal the exact regions required for the elongation-stimulatory function of Elongin and elucidate the mechanism of elongation stimulation by Elongin. Results Stimulation of Pol II elongation by recombinant human Elongin We prepared human Elongin (Fig. ...
Structure and Mechanism of Yeast RNA Triphosphatase Christopher D. Lima, Li Kai Wang, Stewart Shuman Cell Volume 99, Issue 5, Pages 533-543 (November 1999) DOI: 10.1016/S0092-8674(00)81541-X Figure 1 Structural Conservation among Fungal RNA Triphosphatases The secondary structure of S. cerevis...
Structure of DNA Ligase ATP-dependent ligases (based on Bacteriophage T7) NAD+ dependent ligases (based on E. coli LigA ligase) Types of DNA Ligase ATP dependent DNA ligases: NAD+ dependent DNA ligases: Mechanism of DNA ligase 1. Adenylation of DNA ligase/ Activation of DNA ligase ...
Structure-function analysis of Methanobacterium thermoautotrophicum RNA ligase--engineering a thermostable ATP independent enzyme.Structure-function analysis of Methanobacterium thermoautotrophicum RNA ligase--engineering a thermostable ATP independent enzyme.Background: RNA ligases are essential reagents for many...
Bonne D, Pantaloni D (1982) Mechanism of tubulin assembly: guanosine 5′-triphosphate hydrolysis decreases the rate of microtubule depolymerization. Biochemistry 21: 1075–1081 PubMedCASGoogle Scholar Bordas J, Mandelkow EM, Mandelkow E (1983) Stages of tubulin assembly and disassembly studied by ...
Transcription initiation requires assembly of the RNA polymerase II (Pol II) pre-initiation complex (PIC) and opening of promoter DNA. Here, we present the long-sought high-resolution structure of the yeast PIC and define the mechanism of initial DNA opening. We trap the PIC in an intermediate...