Using MDCK-MDR1 cells (a transfected cell line overexpressing human P-glycoprotein (multidrug resistance 1; MDR1)) to evaluate whether your compound is a substrate of the P-glycoprotein (P-gp) efflux transporter is crucial in early drug discovery/development as such knowledge feeds into both: ...
Preparation of MDR1-MDCK Cells Figure 2. Morphology of MDR1-MDCK cells. Method and Results Compounds are applied to the apical or basolateral side of the cell monolayer. The apparent permeability and efflux ratio of the test compounds was determined in duplicate. Compounds were quantified by LC...
MDCK-MDR1细胞药物体外吸收模型的建立及验证
有报道∞引,经转染了人 的稳定,这一作用部分是通过脑微血管内皮细胞 mdrl基因的MDCK细胞内的神经酰胺己三糖苷含 microvascularendothelial (brain cells,BMECs)上 P-gp的外排作用实现的。BBB的体外模型BMECs体,从而使得MDCK—MDRl细胞对细菌外毒素的敏 I astrocytes,TIA)体外共感性增加了1×105—1×106倍。 与I...
(brainmicrovascularendothelialcells,BMECs)上 P—gP 的外排作用实现的.BBB 的体外模型BMECs 与I 型星形胶质细胞(typeIastrocytes,TIA)体外共 培养易丢失部分甚至全部的生理学特性,并且生存 时间短,传代次数少.而MDCK—MDR1 细胞是作为 BBB 药物透过快速筛选的理想模型¨.Madgula 等以MDCK—MDR1 细胞为...
(P-gp) expression in MDCK-MDR1 cells,it could be used as a drug transport model to screen P-gp substrates or inhibitors,as well as models of intestinal mucosa,blood brain barrier and kidney.Although there are some disadvantages in MDCK-MDR1 cell model,it is an effecti...
英文名称MDR1 transfection into canine kidney cells MDCK-MDR1 英文同义词MDR1 transfection into canine kidney cells MDCK-MDR1 CAS号 分子式 分子量0 EINECS号 相关类别 Mol文件Mol File 结构式 MDR1转染犬肾细胞MDCK-MDR1 性质 MDR1转染犬肾细胞MDCK-MDR1 用途与合成方法 ...
(P—gP)expressioninMDCK—MDRlcells,itcouldbeusedasadrugtransportmodeltoscreenP-gPsubstratesorinhibitors,aswellasmodelsofintestinalmucosa,bloodbrainbarrierandkidney.AlthoughtherearesomedisadvantagesinMDCK-MDRlcellmodel,itisaneffectivetoolforimplementingpermeabilityofdruginanoptimalfashion.Keywords:MDCK-MDRlcell;P—...
CAS号: 英文名: MDR1 transfection into canine kidney cells MDCK-MDR1 英文别名: MDR1 transfection into canine kidney cells MDCK-MDR1 中文名: MDR1转染犬肾细胞MDCK-MDR1 中文别名: MDR1转染犬肾细胞MDCK-MDR1 CBNumber: CB48002572 分子式: 分子量: 0 MOL File: Mol file ...
克隆类型:Poolcells 种属来源:犬 组织来源:犬肾 疾病特征:—— 细胞形态:上皮细胞样 生长特性:贴壁生长 培养体系:DMEM-H+10% FBS+1% P/S 配套培养基货号:TCO-G043 传代比例:1:2-1:3,每2-3天换液一次 传代周期:24-48 h 培养条件:气相:95%空气+5%CO2,温度:37℃ 冻存条件:60%基础培养基+30%FBS+...