在真核生物中,负责在复制过程中解开DNA双链的酶最初被康奈尔大学的Bik-Kwoon Tye教授在1983年确定为来自酿酒酵母的小染色体维持蛋白复合物(minichromosome maintenance protein complex, MCM)基因。六个MCM基因--- MCM2至MCM7(MCM2-7)---的产物形成一种六亚基的环状复合物,作为DNA解链机器---DNA复制螺旋酶---...
在真核生物中,负责在复制过程中解开DNA双链的酶最初被康奈尔大学的Bik-Kwoon Tye教授在1983年确定为来自酿酒酵母的小染色体维持蛋白复合物(minichromosome maintenance protein complex, MCM)基因。六个MCM基因--- MCM2至MCM7(MCM2-7)...
Our studies warrant further exploration of design and screening for MCM2-7 complex-specific inhibitors.doi:10.1158/1538-7445.AM2021-1167Tanya Ivanova StoyanovaAmerican Association for Cancer Research (AACR)Cancer research
MCM2-7 complex is a novel druggable target for neuroendocrine prostate cancerEn-Chi Hsu, Michelle Shen, Merve Aslan, Shiqin Liu, Manoj Kumar, Fernando Garcia-Marques, Holly M. Nguyen, Rosalie Nolley, Sharon J. Pitteri, Eva Corey, James D. Brooks & Tanya Stoyanova ...
The MCM2–7 complex is essential for both the initiation and elongation phases of eukaryotic chromosome replication. There is some evidence that MCM2–7 proteins may act as a DNA helicase; at the same time, a variety of other DNA helicases have also been implicated in the replication of euk...
Although there is evidence that individual components might have other roles, the essential nature of the MCM2-7 complex in DNA replication has made it difficult to uncover these. Here, we present a detailed analysis of Arabidopsis thaliana mcm2 - 7 mutants and reveal phenotypic differences. The...
Moreover, the proximity of the MCM2-7 complex to the B2-motif highlights that the loaded helicase remains predominantly static on DNA once the DNA licensing reaction has concluded. Although the B2 sequence motif is not as well defined as the A- element, we wondered whether the underlying B2...
The MCM2–7 complex is believed to function as the eukaryotic replicative DNA helicase. It is recruited to chromatin by the origin recognition complex (ORC), Cdc6, and Cdt1, and it is activated at the G1/S transition by Cdc45 and the protein kinases Cdc7 and Cdk2. Paradoxically, the ...
A heterohexameric complex composed of minichromosome maintenance protein 2–7 (MCM2–7), which acts as a key replicative enzyme in eukaryotes, is crucial for initiating DNA synthesis only once per cell cycle. The MCM complex remains inactive through the G1 phase, until the S phase, when it ...
Origin recognition complex (ORC)-dependent loading of the replicative helicase MCM2-7 onto replication origins in G1-phase forms the basis of replication fork establishment in S-phase. However, how ORC and MCM2-7 facilitate genome-wide DNA licensing is not fully understood. Mapping the molecular...