(2013). Ciprofloxacin is an inhibitor of the Mcm2-7 replicative helicase. Biosci. Rep. 33.N. Simon, M. L. Bochman, S. Seguin, J. L. Brodsky, W. L. Seibel, and A. Schwacha, "Ciprofloxacin is an inhibitor of the Mcm2- 7 replicative helicase," Bioscience Reports, v...
Ciprofloxacin is an inhibitor of the Mcm2-7 replicative helicase. Biosci. Rep. 33(5), e00072. https://doi.org/10.1042/BSR20130083 (2013). Article CAS PubMed PubMed Central Google Scholar Mosquera, J. M. et al. Concurrent AURKA and MYCN gene amplifications are harbingers of lethal tre...
In contrast, actinomycin D, an inhibitor of the primase associated with DNA polymerase α (44), led to a large stimulation of Cdc45 binding (Fig. 7A, compare lanes 1 and 2). Importantly, in the presence of actinomycin D, Cdc45 binding was still completely dependent on the presence of ...
In contrast to a Cdc7 kinase inhibitor, this mode of inhibition may allow increased specificity for the inhibition of helicase activation. Methods Protein purification ORC, Cdc6, Cdt1, and MCM2-7 were purified as previously described23. Plasmids generated in this study are described in ...
Trichostatin A (TSA), a classical HDAC inhibitor, has bee... Y Liu,G He,Y Wang,... - 《Toxicology Letters》 被引量: 69发表: 2013年 Phosphorylation of MCM3 protein by cyclin E/cyclin-dependent kinase 2 (Cdk2) regulates its function in cell cycle. MCM2-7 proteins form a stable ...
In this review, we summarize the currently available data regarding the regulatory mechanisms and functional consequences of MCM phosphorylation and seek the probability that protein kinase inhibitor can be used therapeutically to target MCM phosphorylation in cancer....
In this review, we summarize the currently available data regarding the regulatory mechanisms and functional consequences of MCM phosphorylation and seek the probability that protein kinase inhibitor can be used therapeutically to target MCM phosphorylation in cancer. 展开 关键词:...
Interactions between the CMG components were observed only after the G1/S transition of the cell cycle and were abolished by treatment of cells with either a CDK inhibitor or siRNA against the Cdc7 kinase. Stable association of CMG required all three components of the CMG complex as well as ...
Treatment of cells with low concentrations of CDC7 inhibitor TAK-931 sensitized resistant cells to Vemurafenib and reduced the number of cell colonies. Taken together, CDC7 overexpression and downregulation of miR-3613-3p were associated with Vemurafenib resistance in BRAF- bearing melanoma cells. ...
Cells were lysed in cytoskeleton extraction buffer (CSK) (100 mM NaCl, 10 mM PIPES, pH 7, 300 mM sucrose, 3 mM MgCl2, 0.1% (v/v) NP-40 with protease inhibitor cocktail and phosphatase inhibitors (10 mM NaF, 1 mM orthovanadate) at 4 °C for 20 min. Lysates were then centrifuged ...