A: MCF_7 cells negative for the expression; B: MCF_7 cells transfected with pcDNA 3.1_HER2 positive for HER2 expression, with the membrane stained brown 图4 HER2 在细胞膜上表达的流式细 胞仪检测 i .4 low ytom tri analysis or sur a xpr ssion o $%&2 ...
HER2positive cases account for 1525% of breast cancer cases, and one of the first events in breast carcinogenesis is HER2 upregulation. Furthermore, HER2 expression increases the detection rate of metastatic or recurrent breast cancers by 5080%. The epidermal growth factor recepto...
MCF-7 breast cancer cells are estrogen E2-sensitive cells expressing high levels ofERαtranscripts and very low levels ofERβ.MCF7 cells increase expression of estrogen receptor (ER) in the absence of estrogens. Response to E2 of MCF7 cells was demonstrated to be dependent on autocrine factor ...
Moreover, although LumA tumours had similar cell adhesion-related gene expression compared to normal tissues, MCF7 showed reduced cell adhesion-related gene expression compared to MCF10A. These findings suggest that MCF7 and MCF10A cell lines were limited in their ability to model cancer-related ...
HOXB8 and HOXB9 expression profiles in cell lines extensively used in the literature addressing the putative role of HOXB genes in breast cancer (MCF7, BT474, SKBR3, MDA231 and MDA468) and representative of the clinical breast cancer molecular subtypes (Luminal A, Luminal B, HER2+ and Tripl...
receptor (EGFR) or HER3 『21.HER2 oncoprotein overex— pre ssion is oft en correlated w ith the grow th.metastasis. and therapeutic resistance 『3,4 1.A ccumul ated clinical and lab oratory evidences h ave indicat ed that ov erexpression Of HE R2 /neu may re nder tunqor cells...
represents the HER2 subtype (5%), the database generally coincides with the reported population prevalence [31]. It is important to recognize that gene expression in tumor samples, such as those described in Fig.7, does not imply that GR expression is restricted to tumor cells; the GR could...
Interestingly, we found higher expression of ATP13A4 in the MCF7 cells as compared to the MCF10A cells, which contributed to increased polyamine uptake and cytotoxicity, as well as the activation of MAPK signaling. Our study is the first to characterize ATP13A4 as a polyamine transporter that...
DDC expression was analyzed by immunohistochemistry. The effect of epinephrine on the expression of DDC and the GiGi- protein was analyzed on the protein level via Western blot. A viability assay was performed to test the metabolic cell viability. The overexpression of DDC in the primary tumor ...
Similarly, it was discovered that well-differentiated breast carcinomas have higher levels of ER expression than poorly differentiated breast carcinomas [115]. Conversely, ER-low breast tumours were linked to increased Ki-67 expression, negative PR expression, positive HER2 expression, larger size, an...