Transfection of NS-1 Mammalian Cells with pBI-CMV5 Vector and Cell Culture Procedure DesignTo avoid cell line contamination, all the procedures were performed in a sterile hood which interiorsurfaces were disinfected with 70% ethanol; all the pipettes and pipette tips were autoclaved;and all the ...
The integration of physical and cell culture characterization allows the comprehensive assessment of cell culture performance. The presented approach can serve as a general procedure to develop a scalable mammalian cell perfusion culture. 展开 关键词: Bioprocess design Bioreactors Animal cell culture ...
Tissue culture offers a unique possibility for combining the advantages of experimentally well-controlled in vitro conditions with the advantage of working with living cells. This chapter describes the technical procedure for obtaining reproducible primary nerve cell cultures. The close interrelations among ...
The procedure described in the chapter is a further modification of this technique. Large numbers of viable glomeruli that can be maintained in cell culture can be obtained with this method. In this method, the kidneys are carefully removed using sterile technique. The capsule is stripped from ...
Hydroxychloroquine blocks SARS-CoV-2 entry into the endocytic pathway in mammalian cell culture Zixuan Yuan, Mahmud Arif Pavel, Hao Wang, Jerome C. Kwachukwu, Sonia Mediouni, Joseph Anthony Jablonski, Kendall W. Nettles, Chakravarthy B. Reddy, Susana T. Valente & Scott B. Hansen...
PROCEDURE Four trials of 8 weeks duration were completed. In each trial seventy-five males ... Glick,B. - 《Poultry Science》 被引量: 22发表: 1958年 Differential Effect of Procaine on Irradiated Mammalian Cells in Culture1 Djordjevic B. Differential effect of procaine on irradiated mammalian ...
7a). We first swapped the miRBSs of the previous classifier (containing miR-21 MITA and miR-18a MITR) to generate a new classifier with miR-18a MITA and miR-21 MITR, expecting this to confer HEK293 cell-targeting cytotoxicity. We then performed LDH assays and co-culture flow cytometry ...
As I major in molecular biology, there are losts of experiments I need to do every day. Here I will demonstrate a fundamental experiment, cell passaging. Passaging or subculturing of cells is a common procedure wherein cells from a given culture are divided, or “split”, into new cultu...
A dot immunoblotting technique has been developed to estimate the relative expression levels of tagged recombinant human proteins in mammalian cell culture media. Variations in sample denaturation, blocking agents and membrane composition and treatment were used to optimize the signal-to-noise ratio of ...
Cell culture The HEK293FT cell line was purchased from Thermo Fisher/Life Technologies (RRID: CVCL_6911) and was not further authenticated. The HEK293FT-LP cell line was a gift from Ron Weiss and was authenticated by flow cytometric analysis of EYFP expression, which was shown to be homogeno...