Towards quantitative metabolomics of mammalian cells: Development of a metabolite extraction protocolMetabolomicsMammalian cell cultureQuenchingExtractionMetabolomics aims to quantify all metabolites within an organism, thereby providing valuable insight into the metabolism of cells. To study intracellular ...
Pistikopoulos E.N., Mantalaris A., Cell Cycle modelling for Off-line dynamic optimisation of mammalian culture, Computer Aided Chemical Engineering, v. ... Al-Rubeai,Mohamed 被引量: 0发表: 2015年 A subcellular prefractionation protocol for minute amounts of mammalian cell cultures and tissue...
in Animal Cell Culture 27–64 Springer, 2015). Vazquez-Lombardi, R. et al. Potent antitumour activity of interleukin-2-Fc fusion proteins requires Fc-mediated depletion of regulatory T-cells. Nat. Commun. 8, 15373 (2017). Article CAS Google Scholar Kontermann, R.E. Dual targeting ...
Mammalian cell cultureserum-free mediumsuspension cultureadaptationbiopharmaceuticalbioreactorA three-step protocol is described for adapting an anchorage-dependent, ... MS Sinacore,D Drapeau,SR Adamson - 《Molecular Biotechnology》 被引量: 195发表: 2000年 SERUM-FREE CULTURE MEDIUM FOR MAMMALIAN CELLS AN...
First, the influence of continuous illumination during cell culture was comparatively investigated for white, red, and blue light (Fig. 1a) of equal intensity in contrast to the dark control. After seeding the cells into cell culture plates, they were allowed to attach in the dark. 24 h ...
In this technique, cells are seeded in a chamber of known height and a fluorescent probe that does not enter the cell is added to the culture media. The fluorescence intensity is negatively proportional to the height of the cell and the exact volume of the cell can therefore be calculated ...
Imax represents the largest current response obtained from a voltage step for each recorded cell. Patch-clamp experiments conducted on primary dissociated cortical neurons followed a modified protocol. Neurons were allowed to mature for 11–14 days in vitro prior to recording. Compared to HEK cells,...
were used for the construction of stable cell lines. Transfection was performed according to the manufacturer’s protocol. The EYFP-Huh7 cell line was derived from Huh7 cell line by transfection with the lenti-GF1-CMV-EYFP lentivirus. The mScarlet-HEK293 cell line was derived from HEK293 cell...
Cell culture and transfection HEK293 (293-H) cell line was purchased from Life Technologies. Hela S3 cell line was purchased from ATCC. HEK293 or HeLa S3 cells were cultured in high-glucose DMEM complete media (Dulbecco’s modified Eagle’s medium (DMEM), 4.5 g l−1 glucose, 0.045...
With regard to mammalian cell genotoxicity tests, we now understand much better when a positive result may be misleading,i.e.not predictive of carcinogenic orin vivomutagenic activity, and a number of protocol-driven artefacts leading to positive responses have been identified,e.g., high osmolality...