Mainly forward-genetic screens were used in cell culture cells, which rely on the mutagenesis of the organisms (usually through DNA transfection), the recognition of the altered phenotype or biochemical changes and the determination of the causative DNA element. ...
This experiment involves the growth of mammalian cell cultures established from embryos of pregnant mice. Growth parameters will be varied to determine the effect of each on cell growth. The purpose of this experiment is to determine the most economical way to establish and maintain a mouse cell ...
Proteins expressed inmammalian cellculture systems can undergo complexPTMsby copying nature, a significant advantage over other systems that are limited to high-mannoseglycoproteinmodifications (baculovirus/insect cell system and yeast) or incapable of any type of PTM (bacteria). Thus, the assembly of...
In this technique, cells are seeded in a chamber of known height and a fluorescent probe that does not enter the cell is added to the culture media. The fluorescence intensity is negatively proportional to the height of the cell and the exact volume of the cell can therefore be calculated ...
The molecular mechanisms that underlie the establishment of autonomous replicons in long-term mammalian cell culture are unknown, despite the potential importance of ectopic gene expression in biomedicine [19]. Towards understanding this mechanism, we have performed a detailed study of the interaction of...
A system and method for controlling a mammalian cell culture process are provided. Such control of the cell culture process involves control of the level of dissolved carbon dioxide in the cell cultur
it seems likely that we will improve our ability to conductin vitroassays reproducibly and apply data obtained in cell lines to human patients (Goodspeed et al., 2016). We note that RNAi, CRISPR, and other perturbational experiments in which phenotypes are measured in cell culture are likely ...
in Animal Cell Culture 27–64 Springer, 2015). Vazquez-Lombardi, R. et al. Potent antitumour activity of interleukin-2-Fc fusion proteins requires Fc-mediated depletion of regulatory T-cells. Nat. Commun. 8, 15373 (2017). Article CAS Google Scholar Kontermann, R.E. Dual targeting ...
A chemically defined mammalian cell culture medium is provided that supports maintenance and long term clonal growth of mammalian hepatocytes and other cells.
1. A method for differentiating pluripotent mammalian cells, the method comprising: (I) culturing pluripotent mammalian cells to obtain a cell population at least partially differentiated into endoderm lineage cells, wherein the culturing is performed a cell culture chamber comprising a plurality of mic...