A novel 8-gene signature was constructed to predict prognosis based on 465 macrophage marker genes identified by an analysis of single-cell RNA sequencing data of LUAD, and was also verified in 4 independent GEO cohorts. The MMGS significantly classified patients into high-risk and low-risk ...
and MAPK pathways are also involved in M1 and M2 polarization. The JAK/STAT pathway is activated by cytokines such as IFN-γ and IL-4 and promotes the activation of STAT1 and STAT6, respectively. The PI3K/Akt pathway is activated in M2 macrophages and promotes M2-associated gene expression ...
Based on the gene expression level of each sample in the GSE32707 dataset, the enrichment level of 28 immune cells in each sample in the dataset was analysed using the ssGSEA method in R language. The corrplot package was used to analyse the relationship between the individual immune cell lev...
It is now appreciated that the M2 group encompasses a functionally diverse group of macrophages that can be further subdivided into four subsets; M2a, M2b, M2c, and M2d based on their distinct gene expression profiles. As shown in table 5 below, the M2a subtype is elicited by IL-4, IL-...
Both PKM2I428&429A and PKM2R399E functioned in ectosome-induced gene expression of differentiation-associated transcription factors and macrophage differentiation as effectively as PKM2WT, but PKM2I267&268A failed to exert the same effects (Figures 5E–5H). Thus, the regulation of monocyte-to-...
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The following table provides a broad list of proteins expressed by macrophages, as mentioned by various sources. They are categorized by their human NCBI gene symbol (Gene), common names (Synonyms), expression in human or mouse (Species), type of macrophage marker (Marker Type), protein type,...
We normalized gene expression to Gapdh levels and calculated the relative levels using 2-ΔΔCt. All reactions were performed in triplicates. The primers were designed and synthesized by Sangon Biotech (Shanghai, China) as follows: Gapdh: forward primer 5ʹ-ACA GCA ACA GGG TGG TGG AC-3...
We then genetically ablated the Mdh1 gene by transducing BMDMs from LysM-Cre Cas9 mice with lentiviruses expressing an sgRNA targeting Mdh1 (Extended Data Fig. 6g). We found that FGK45 treatment failed to induce the expression of pro-inflammatory marker genes and the production of cytokines in ...
we defined gene signatures characteristic of macrophages, T cells and B cells using 4–5 hallmark genes of these cell types (Supplementary Table1). As expected, these signatures projected to matching cell-types in scRNA-seq datasets of RLTs (Supplementary Fig.2) and DLBCL (Supplementary Fig.3)...