intraperitoneal (i.p) injection of 20 mg/kg O55:B5 LPS and treated with either 2 g/kg mannose or saline control (vehicle) solution given hourly by i.p. injections for up to six times. Incmice were sacrificed after 3 h and IL-1β concentration in serum determined by ELISA. Dat...
results.H,IProtein level of IL-1β (H), IL-18 (I) fromHif2α+/+and LysMHif2αLSL/LSLBMDMs treated with So(d18:1) or not under NLRP3 inflammasome stimulation. (n = 6 biologically independent samples). Data are the means ± s.e.m.B,E,H,IStatistical analysis was ...
CCL4 and CCL2 (ELISA)Suppression of activated T cell proliferation (in vitro) scRNA-seq Fluidigm C1 CITE-seq [52,114] P2RY12+, CX3CR1+, CD49D- (FACS) HLA-DRint, BIN1+, TMEM119+ (IHC), CD69+, CD151+, TMEM119+ (CITE-seq) Mouse Bin1, Cst7, Cxcl13, Hexb, Nav3, P2ry12,...
(n = 5; scale bar, 50 μm); F Representative immuno- histochemical analyses of TNFα in murine kidney of each group (n = 5; scale bar, 100 μm); G Real-time qPCR showing mRNA levels of Tnfa, Il1b and Il6 in the kidney tissues; H Representative western blot ...
M1 macrophage activation occurs through signaling by IFNG, TNF, and toll-like receptors. Genetic markers associated with M1 polarization include IL1a, IL1b, IL6, NOS2, TLR2, TLR4, CD80, and CD86. For M2 macrophages, activation occurs through cytokines including IL4, IL10, and IL13. Report...
Retinal detachment (RD) induces activation of IL-1β, which is diminished inRip3-deficient mice. (a) The data show IL-1βlevels detected by ELISA at 0 h (non-treated eyes,n=6) and from 6 to 120 h after induction of RD.n=6–9. (b) Western blotting image shows representative...
However, miR-155 antagomir transfection resulted in no change of intracellular levels of three miR-155 known targets, namely Socs1, Ship1 and Sirt1 neither under basal conditions nor after up-regulation induced by LPS (Fig. 3F). Similarly, Il6 and Il1b up-regulation was not altered by mi...
The extent of the inflammatory response was assessed by the secretion of cytokines CCL2, IL8, IL6, IL1尾, and TNF measured by ELISA. Results: Basal level of secretion of cytokines CCL2, IL8 and TNF was 1.5-2 times higher in cultures of cybrids with defective mitophagy compared to cells ...
This subset is significantly enriched in the immune microenvironment of bone-invasive PitNETs and exhibits high expression of TNF-α and IL1B. The TNF-α+TAM may be functionally related to lymphocyte activation, inflammatory response, TNF signaling pathway, and osteoclast differentiation, potentially ...
injected intraperitoneally (i.p.) with LPS (0,1 mg/kg of body weight)E.coliO55:B5 (Sigma-Aldrich). Four hours after the LPS injection, blood was taken and sera prepared. Mouse IL-6 and mouse IL-10 cytokines were determined using ELISA (Invitrogen) according to the manufacturers ...