TRAC-Seq在原理上相似,只是前者使用总RNA作为起始材料,而后者使用小RNA作为起始材料,并加入AlkB去甲基化和硼氢化盐还原步骤,从而实现tRNA高效逆转录。上述四种化学方法都以单核苷酸分辨率检测m7G修饰位点,但由于m7G位点的部分还原、脱氨和裂解,其准确性仍然较差。为此,Zhang等人进一步优化m7G-Seq,开发了m7G-quant-Seq,实...
In addition, there are also many m7G modification sites inside mRNA [28]. Zhang et al. found that the m7G cap was removed, and the proportion of m7G in mammalian mRNA was 0.02%-0.05%. At the same time, based on the method of MERIP-seq and reverse transcription, a new sequencing metho...