QuantSeq is the gold-standard solution for gene expression studies targeting the 3’ end of RNA transcripts. You can work with very low inputs, degraded samples, or blood – QuantSeq will be your companion of choice during your RNA-Seq journey! Please see additional benefits by clicking on ...
QuantSeq Kit 是一个文库制备试剂盒,由它构建的文库可以用于Illumina和总RNA的起始量低至100 pg(对于Illumina平台)和5ng( 对于Ion Torrent平台)。QuantSeq适用于反复构建低质量的RNA文库,包括FFPE样品。 Rapid Turnaround 简单的工作流程可以将文库制备时间控制在4.5小时以内,而这已经包括了手工操作时间。除去手工操作...
QuantSeq试剂盒的可选Globin Block (RS-Globin Block)模块能够在文库制备过程中,通过阻止其第二条链的扩增,避免球蛋白mRNA片段生成。该模块只与QuantSeq 3’ mRNA-Seq Library Prep Kits for Illumina (FWD, 目录号015, 和 REV, 目录号016)兼容,适用于血液RNA样本制备文库。 每个模块提供一个改进的RNA去除溶液(...
Our calculator helps you determine the volumes you need to mix when pooling samples together in your QuantSeq-Pool experiment. Try it now!
QuantSeq is an oligodT primed NGS library preparation protocol generating only one fragment per transcript. This enables accurate gene expression quantification independent of the RNA quality (including FFPE samples). Standard mRNA-Seq protocols aim to cover the whole transcript, and result in a ...
QuantSeq建库试剂盒提供了文库构建的实验方案,可构建适用于Illumina和Ion Torrent系统的、靠近poly(A) RNA 3' 端序列的文库。每个转录物仅生成一个片段,从而准确的实现了基因表达的量化,同时节省了测序空间并实现了高度的多重性(saving on sequencing space and allowing for a high level of multiplexing)。
QuantSeq provides an easy protocol to generate highly strand-specific next-generation sequencing (NGS) libraries close to the 3′ end of polyadenylated RNAs within 4.5 h. Only one fragment per transcript is generated, directly linking the number of reads mapping to a gene to its expression. Qu...
yarn add jbrowse-plugin-quantseq Usage Add to the "plugins" of your JBrowse Web config: {"plugins": [ {"name":"Quantseq","url":"https://unpkg.com/jbrowse-plugin-quantseq/dist/jbrowse-plugin-quantseq.umd.production.min.js"} ] } ...
RNA-Seq is increasingly used for the diagnosis of patients, targeting of therapies and for single cell transcriptomics. These applications require cost effective, fast and reliable ways of capturing and analyzing gene expression data. Here we compared Lexogen’s QuantSeq which captures only the 3′...
RNA-Seq quantification using a rescuing scheme that distributes ambiguously mapped reads based on uniquely mapped ones. - Yue-Jiang/RNASeqQuant