对于 Illumina 的QuantSeq3' mRNA-Seq 文库制备试剂盒中的barcodes允许在 Illumina flow cell每条通道上测序的样本多达96个。对于Ion Torrent的QuantSeq3' mRNA-Seq文库制备试剂盒中的barcodes允许在Ion Torrent仪器上多达48个样本一起进行测序。如此高的混样程度可以节约成本如同QuantSeq特定长度节省测序空间一样。
Standard mRNA-Seq protocols aim to cover the whole transcript, and result in a heavy 3' bias when used on degraded RNA. Comparison between high quality and low quality RNA samples is therefore hampered and often biased. QuantSeq 3' mRNA-Seq focuses on counting 3' ends and therefore ...
QuantSeq 3′ mRNA-Seq (Illumina) QuantSeq 3′ mRNA-Seq FWD with UDI 12 nt Kits (Unique Dual Indexing) Version 2: Use for Cat. No. 191, 194, 195, 196, 193, or 192 (QuantSeq 3’ mRNA-Seq V2 Library Prep Kit FWD with UDI 12 nt, Sets A1, A2, A3, A4, A1 – A4, or B1)...
The QuantSeq libraries were prepare using Lexogen’s QuantSeq 3′ mRNA-Seq Library Prep Kit for Illumina, according to the manufacturer’s instructions. The QuantSeq libraries were sequenced using the Illumina NextSeq 500 to produce 75 bp single-end reads for each sample. Library preparation ...