对于 Illumina 的QuantSeq3' mRNA-Seq 文库制备试剂盒中的barcodes允许在 Illumina flow cell每条通道上测序的样本多达96个。对于Ion Torrent的QuantSeq3' mRNA-Seq文库制备试剂盒中的barcodes允许在Ion Torrent仪器上多达48个样本一起进行测序。如此高的混样程度可以节约成本如同QuantSeq特定长度节省测序空间一样。
Standard mRNA-Seq protocols aim to cover the whole transcript, and result in a heavy 3' bias when used on degraded RNA. Comparison between high quality and low quality RNA samples is therefore hampered and often biased. QuantSeq 3' mRNA-Seq focuses on counting 3' ends and therefore ...
Use for Cat. No.s: 222.24/.96/.4×96 (QuantSeq FFPE 3‘ mRNA-Seq Library Prep Kit FWD with UDI 12 nt Set A1, or A1-A4) and 223.24/.96 (QuantSeq FFPE 3‘ mRNA-Seq Library Prep Kit FWD with UDI 12 nt Set B1) Current Version: ...
图1 | QuantSeq 3’ mRNA-Seq建库试剂盒(左侧)和Quant-Seq-Flex建库试剂盒(右侧) 工作流程图。QuantSeq FWD试剂盒(Cat No. 015)的Read 1反映了靠近3‘端的mRNA序列,实现了Illumina标准测序引物的经济的二代测序。在QuantSeq REV试剂盒中(Cat. No. 016),Read 1和Read 2的接头(adapter)位置互换,从而实现...
Easily determine the volumes you need to mix when pooling samples together in the QuantSeq-Pool Sample-Barcoded 3’ mRNA-Seq Library Prep Kit for Illumina kit (Cat. No. 139), thanks to our user-friendly QuantSeq-Pool Calculator.This tool also provides you with recommended input range (...
The QuantSeq libraries were prepare using Lexogen’s QuantSeq 3′ mRNA-Seq Library Prep Kit for Illumina, according to the manufacturer’s instructions. The QuantSeq libraries were sequenced using the Illumina NextSeq 500 to produce 75 bp single-end reads for each sample. Library preparation ...