对于 Illumina 的QuantSeq3' mRNA-Seq 文库制备试剂盒中的barcodes允许在 Illumina flow cell每条通道上测序的样本多达96个。对于Ion Torrent的QuantSeq3' mRNA-Seq文库制备试剂盒中的barcodes允许在Ion Torrent仪器上多达48个样本一起进行测序。如此高的混样程度可以节约成本如同QuantSeq特定长度节省测序空间一样。
Standard mRNA-Seq protocols aim to cover the whole transcript, and result in a heavy 3' bias when used on degraded RNA. Comparison between high quality and low quality RNA samples is therefore hampered and often biased. QuantSeq 3' mRNA-Seq focuses on counting 3' ends and therefore ...
图1 | QuantSeq 3’ mRNA-Seq建库试剂盒(左侧)和Quant-Seq-Flex建库试剂盒(右侧) 工作流程图。QuantSeq FWD试剂盒(Cat No. 015)的Read 1反映了靠近3‘端的mRNA序列,实现了Illumina标准测序引物的经济的二代测序。在QuantSeq REV试剂盒中(Cat. No. 016),Read 1和Read 2的接头(adapter)位置互换,从而实现...
The QuantSeq 3’ mRNA-Seq Library Prep method generates Next Generation Sequencing (NGS) libraries from single sites close to the poly(A) tail of RNAs. Due to its fast and highly sensitive workflow this tag profiling method is ideal for the accurate quantification of gene expression, also from...
Animal fats are considered to be unhealthy, in contrast to vegetable fats, which are rich in unsaturated fatty acids. However, the use of some fats, such as coconut oil, is still controversial. In our experiment, we divided experimental animals (domestic