m6A reader protein YTHDF2 regulates spermatogenesis by timely clearance of phase-specific transcriptsm6Amale sterilitymRNA clearancespermatogenesisYthdf2Accumulating evidences show that the regulatory network of m6A modification is essential for mammalian spermatogenesis. However, as an m6A reader, the roles ...
13 Chen, C. et al. Nuclear m(6)A reader YTHDC1 regulates the scaffold function of LINE1 RNA in mouse ESCs and early embryos.Protein Cell, doi:10.1007/s13238-021-00837-8 (2021). 14 Xiao, W. et al. Nuclear m(6)A Reader YTHDC1 Regulates mRNA Splicing.Mol Cell61, 507-519, doi:...
N6-甲基腺苷(N6-methyladenosine,m6A)修饰是真核生物mRNA转录后修饰中最常见、最丰富的化学修饰之一,由“Writer”甲基转移酶复合体(包括METTL3、METTL14及辅助因子WTAP等),“Reader” RNA阅读蛋白(包括YTH域家族YTHDF1-3、YTHDC1-2和I...
2021年3月16日上海交通大学医学院附属第九人民医院的贾仁兵教授团队在Genome Biology上发表题为Histone lactylation drives oncogenesis byfacilitating m6A reader protein YTHDF2expression in ocular melanoma的文章,该研究揭示了组蛋白乳酰...
N6-甲基腺苷(N6-methyladenosine,m6A)修饰是真核生物mRNA转录后修饰中最常见、最丰富的化学修饰之一,由“Writer”甲基转移酶复合体(包括METTL3、METTL14及辅助因子WTAP等),“Reader” RNA阅读蛋白(包括YTH域家族YTHDF1-3、YTHDC1-2和IGF2 RNA结合蛋白家族IGF2BP1-3等),以及“Eraser”去甲基化酶(FTO和ALKBH5)共...
在体内,m6A修饰主要被三类蛋白分子调节,催化在RNA上形成甲基化的酶一般称为“编码器”(writer,m6A甲基转移酶),去除在RNA上甲基化的酶被称为“消码器”(eraser,m6A去甲基化酶),识别RNA上甲基化的酶被称为“读码器”(reader,m6A结合蛋白或识别蛋白)[图1]。因此,m6A甲基化修饰是个可逆的动态过程,分别由编码器...
m6A “reader” Ythdf1 binds to Zfp839 and promotes translation of m6A-modifed Zfp839 mRNA We further attempted to confirm whether Zfp839 was regulated by Ythdf1. The mRNA and protein expression levels of Zfp839 in both control and Ythdf1 KO BMSCs were evaluated after 3 days of osteogenes...
2024年5月7日,万建民院士团队与北京大学贾桂芳团队合作在Molecular Plant发表了题为The RNA binding proteinEHD6recruits them6AreaderYTH07and sequestersOsCOL4mRNA into phase-separated ribonucleoprotein condensates to promote rice flowering的研究论文。该论文揭示了RNA结合蛋白通过m6A途径介导的相分离过程调控水稻抽穗...
6. Scutenaire J, Deragon JM, Jean V, Benhamed M, Raynaud C, Favory JJ, Merret R, Bousquet-Antonelli C:The YTH Domain Protein ECT2 Is an m6A Reader Required for Normal Trichome Branching in Arabidopsis.Plant Cell2018,30:986-1005. ...
Furthermore, bioinformatics analysis indicated that the 3' UTR of GLS1 contained a putative binding motif of YTHDF1, and an interaction was further validated by a protein-RNA interaction assay (RNA immunoprecipitation [RIP]). Furthermore, we demonstrated that YTHDF1 promoted protein synthesis of ...