本研究通过视网膜母细胞瘤(RB)细胞和临床样品中的mRNA分离和抗m5C dot blot试验检测全局和mRNA m5C水平。建立原位眼内异种移植模型以检查RB的致癌行为,病进行全基因组多组学分析以鉴定NSUN2的功能靶标,包括蛋白质组学分析,转录组筛选和m5C甲基化RNA免疫沉淀测序(m5C meRIP-seq)。此外进行了基于类器官的单细胞分析和...
本研究通过视网膜母细胞瘤(RB)细胞和临床样品中的mRNA分离和抗m5C dot blot试验检测全局和mRNA m5C水平。建立原位眼内异种移植模型以检查RB的致癌行为,病进行全基因组多组学分析以鉴定NSUN2的功能靶标,包括蛋白质组学分析,转录组筛选和m5C甲基化RNA免疫沉淀测序(m5C meRIP-seq)。此外进行了基于类器官的单细胞分析和...
本研究通过视网膜母细胞瘤(RB)细胞和临床样品中的mRNA分离和抗m5Cdot blot试验检测全局和mRNA m5C水平。建立原位眼内异种移植模型以检查RB的致癌行为,病进行全基因组多组学分析以鉴定NSUN2的功能靶标,包括蛋白质组学分析,转录组筛选和m5C甲基化RNA免疫沉淀测序(m5C meRIP-seq)。此外进行了基于类器官的单细胞分析和基...
dot blot. Quantification of m5C levels (mean ± SD) from three independent experiments normalized with Ctrl and methylene blue is shown.e32P-labeled mRNA monophosphate nucleosides were run on 2D gels for 2D-TLC analysis. In vitro-transcribed 4B mRNA with or without m5C was run in parallel...
RNA m5C dot blot assay Total RNA was first isolated from stable NSUN2 overexpression and knockdown cells and their corresponding negative control cells, and then treated with deoxyribonuclease I (DNase) according to the manufacturer’s protocol. RNA quality was analyzed by NanoDrop 2000 (Thermo Scie...
Initially, we identified significantly elevated global RNA m 5 C levels in both UM cells and tissue specimens using ELISA assay and dot blot analysis. Meanwhile, NOP2/Sun RNA methyltransferase family member 2 (NSUN2) was upregulated in both types of these samples, whereas NSUN2 knockdown ...
本研究通过视网膜母细胞瘤(RB)细胞和临床样品中的mRNA分离和抗m5C dot blot试验检测全局和mRNA m5C水平。建立原位眼内异种移植模型以检查RB的致癌行为,病进行全基因组多组学分析以鉴定NSUN2的功能靶标,包括蛋白质组学分析,转录组筛选和m5C甲基化RNA免疫沉淀测序(m5C meRIP-seq)。此外进行了基于类器官的单细胞分析和...
Dual luciferase reporter assay, m5C‐RIP‐qPCR, and mRNA stability assay were conducted to explore the effect of NSUN2‐depletion on target genes. Cell viability, transwell, and xenograft mouse model were designed to demonstrate the characteristic of NSUN2 in promoting LUAD progression. The m5C ...
The mode of interaction between NSUN6 and NM23-H1 was determined by dot blot, luciferase assay, m5C RIP, and cell function assays. The effect of NSUN6 expression on tumor growth was verified in vivo. Results: Expression of NSUN6 was reduced in lung cancer cells, and ove...
(m5C). The proportion of read signals identified as modified with probability > 0.7 is indicated above each distribution. The box plots in the inset represent the first and third quartiles by the white vertical box, with the median marked by a white dot and whiskers indicated as vertical...