dot blot assay 网络 斑点杂交; 斑点印迹法;[例句]Dot blot assay to DNA of the 12 lines showed that 7 lines had positive hybridization signals.不同株系桑苗叶片DNA点杂交分析显示，7个株系有阳性杂交信号。
Cubie HA, Molyneaux PJ, Shearman MJ, Gryzbowski J, Brown T. Dot-blot hybridisation assay for detection of parvovirus B19 infections using synthetic oligonucleotides. Mol Cell Probes. 1995 Feb; 9 (1):59–65. [ PubMed ]Cubie HA, Molyneaux PJ, Shearman MJ, Gryzbowski J, Brown T. Dot-...
Pools of 10 synthetic oligonucleotides with sequences derived from the genome of parvovirus B19 and of 30 bases in length were made and labelled at the time of synthesis with digoxigenin (DIG) or dinitrophenyl (DNP) at the 5′ end. They were used in a dot-blot hybridisation assay to detect...
To monitor the fate of these toxins in soil, a dot blot enzyme-linked immunosorbent assay (ELISA) that detects free and particle-bound toxins from B. thuringiensis subsp. kurstaki and subsp. tenebrionis was developed. The lower limit of detection of the toxins, either free or adsorbed or ...
Serum HER2 Level Measured by Dot Blot: A Valid and Inexpensive Assay for Monitoring Breast Cancer Progression. Serum HER2 level measured by dot blot:a valid and inexpensive assay for monitoring breast cancer progression. Tan LD,Xu YY,Yu Y,Li XQ,Chen Y,Feng ... Li-Duan,Tan,Yuan-Yuan,.....
A dot blot will provide a qualitative estimate of antibody titer, as described in this protocol. The same test can be done quantitatively by enzyme-linked immunosorbent assay (ELISA), but the technically simpler dot blot gives adequate information, uses less protein, and does not require an ...
A sensitive and specific dot blot immunoassay based on prostate specific antigen (PSA) antibodies and a radiolabelled Protein A detection system was developed for detection of trace amounts of human semen in stains. The method was found highly sensitive and detected semen stain extracts diluted 10...
实验老司机:实验原理:Dot blot是一种半定量的蛋白质或核酸检测方法,其原理是将待测物直接滴于固相载体上,然后通过特异性抗体或探针与待测物结合,最终通过可视化方法来检测目标分子的存在与否。溶液配置:操作步骤:(1)减取适当大小的PVDF膜,于甲醇活化1分钟。 (2)将活化后的PVDF膜于ddH2O中,在脱色摇床上清洗… ...
dot-blot原理(dotblot原理) Immunologicalactivityassay Dotblotprinciple: Principle:antigenantibodyandreceptorligandbindingcanbe usedasasimpleandrapidquantitativeanalysisofproteins. Theantigenantibodyorreceptorligandadsorbedonthebottom ofthe96orificeplatecanbelabeledandquantifiedto identifythebasisforquantitativeanalysis.Beca...
Human epidermal growth factor receptor 2 (HER2) is one of the most important prognostic and predictive factors for breast cancer patients. Recently, serum HER2 ECD level of patients detected by enzyme-linked immunoabsorbent assay (ELISA) has been shown t