dot-blot 原理(dot blot 原理) Immunological activity assay Dot blot principle: Principle: antigen antibody and receptor ligand binding can be used as a simple and rapid quantitative analysis of proteins. The antigen antibody or receptor ligand adsorbed on the bottom of the 96 orifice plate can be...
dot-blot原理(dotblot原理)ImmunologicalactivityassayDotblotprinciple:Principle:antigenantibodyandreceptorligandbindingcanbeusedasasimpleandrap..
dot-blot原理(dot blot原理)Immunological activity assayDot blot principle:Principle: antigen antibody and receptor ligand binding can be used as a simple and rapid quantitative analysis of proteins. The antigen antibody or receptor ligand a
The Dot Blot Assay Where a probe sequence is present in one individual, but absent in another, the gel separation part of the assay can be omitted. Undigestedgenomic DNAis applied directly to the membrane, which is then hybridized with a probe in the normal way. This represents a drastic ...
A Ruthenium-103 Red Dot Blot Assay Specific for Nanogram Quantities of Sulfated Glycosaminoglycans 来自 Elsevier 喜欢 0 阅读量: 25 作者:D J.,Gaffen,and,M F.,Price,and,T M.,Bayliss,and,M R.摘要: Ruthenium-103 red has been used previously to detect nanogram quantities of glycosaminoglycans ...
Here we describe an efficient dot-blot assay for high-throughput screening of two enzymes, heparan sulfate N-deacetylase/N-sulfotransferase (NDST-1) and high-endothelial cell GlcNAc-6-sulfotransferase (HEC-GlcNAc-6-ST). The assay proceeds by transfer of 35S-labeled sulfate from [35S]-3′-phosp...
The host origin of gene segments encoding the internal proteins of H1N1 swine and turkey influenza viruses was identified by developing a dot-blot assay. ... Stephen M. Wright,Yoshihiro Kawaoka,Gerold B. Sharp,... - 《American Journal of Epidemiology》 被引量: 314发表: 1992年 The Role of...
For western blot analysis in human cell lines, expo- nentially growing human leukaemia cell line MV4;11 or Molm13 cells were plated in 6-well plates at 2 Â 105 cells per well in a final volume of 2 ml. Cells were incubated in the presence of DMSO or 10 mM of EPZ004777 or ...
Sandwich immunoassay for the hapten angiotensin II a novel assay principle based on antibodies against immune complexes Immunoassays for haptens such as short peptides or drugs are usually based on the principle of competition for a limited number of binding sites on antibod... OO Zingel - 《Jou...
A ruthe- nium-103 red dot blot assay specific for nanogram quantities of sulfated glycosaminoglycans. Anal Biochem 1994;218:124e30.Gaffen JD,Price FM,Bayliss MT,et al.A ruthenium-103 red dotblot assayspecific for nanogram quantities of sulfated glycosatninoglycans [J].Anal Biochem,1994,218 (...