在小鼠BM、血液和脾脏中,FLT3+CD11c细胞在传统的单核细胞片段中鉴定并表达DCs特异性ICAM-3捕获非整联素(DC-SIGN或CD209a),被认为是单核细胞亚群(R2细胞),产生单核细胞来源的dcMildner等人还发现小鼠血液中的Ly6Cint细胞亚群表达DC特...
其一,(A) Ly6GHi CD11b + 细胞(中性粒细胞); 其二,(B) Ly6CLo - neg Ly6G - SSCHi 细胞(嗜酸性粒细胞)且 MHC Class II - 与 CD115 - (详见附加材料 Fig. 5); 其三,(C) Ly6CLo - neg Ly6G - SSCLo 细胞(单核细胞 / 巨噬细胞)且 MHCII + /- 和 CD115 - (以红色柱状图呈现); 其四,(...
CD44是一种吞噬受体,能够触发巨噬细胞摄入大颗粒或凋亡细胞。MHC-II 能够促进巨噬细胞吞噬和抗原递呈。结果显示这些新生成的CD11b+CD45+细胞高表达CD44和MHC-II(图3B),暗示其具有较强的吞噬功能。CD68是一种单核细胞衍生的巨噬...
We identified Ly6c(low)MHCII(hi) macrophages as the major population promoting the melanocyte response across multiple strains. The activity of this subpopulation was CCR2 (C-C chemokine receptor type 2) independent and partly IL-17 dependent. By helping induce this effect, the infiltration of ...
Using multimarker flow cytometry and RNA expression array analyses on macrophage subpopulations from wound granulation tissue, we identified a Ly6cloMHCIIhi“noninflammatory” subset that increased both in absolute number and proportion during normal wound healing and was missing in Ob/Ob and MYD88-/...
4F-I). Moreover, the percentage of MHC II + macrophages increased (Fig. 4J-K), whereas the percentage of CD163 + macrophages decreased after IRG1 knockout (Fig. 4L-M), which suggested that IRG1 deficiency can skew macrophages towards a pro-inflammatory phenotype. Fig. 4 IRG1...
Pacific orange-conjugated anti-IA/IE; PE-conjugated anti-CD4 or Ly6C; PerCP-Cy5.5–conjugated anti- Ly6C or CD8; PE-Cy7–conjugated mAb to anti-CD11c; allophycocyanin-Cy7-conjugated anti- MHCII, B220 and Ly6G; and allophycocyanin-conjugated anti-CD64 and anti-SIINFEKL/H-2Kb (clone 25...
FACS analysis suggested that decreased Ly6c lo MHCII hi AMs might make the major contribution to attenuated fibrogenesis in RBP‐J cKO mice, probably by reduced inflammatory factor release and enhanced matrix metalloproteinases expression. Using clodronate‐mediated macrophage depletion in RBP‐J ckO ...
In the tumor, M-MDSC can differentiate into tumor-associated macrophages (TAM) (CD11b+ Ly6Clo F4/80+ MHCII+)17. In tumor-bearing host, myeloid cells are recruited to tumor sites by chemokines produced by the tumor, such as CCL2 and CCL518,19. In chronic tumor-associated inflammation, ...
Shortly after transfer, graft-derived cells were retrieved from the inflamed tissue as Ly6ChiCD11c−MHCII+/− cells. Notably, the colon of DSS-challenged mice was reported to harbor two macrophage populations that can be discriminated according to CX3CR1 expression, TLR profiles, and TNF-α...