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Using low-input lipidomics in mouse and human embryos, Zhang, Shui, Li and colleagues find that lipid unsaturation increases with development towards the blastocyst stage. They further show that lipid desaturases contribute to successful embryo implantation.Zhang Ling...
To explore the input limitations of microcentrifuge-based AP-MS workflows, we performed preliminary affinity purification experiments using transgenic HeLa Kyoto cell lines expressing SMC1A as a GFP fusion protein at near-endogenous levels using BAC TransgeneOmics17. SMC1A is a subunit of the human ...