Lipid signatures of different developmental stages are distinct, particularly for the phospholipid classes. We highlight that the high degree of phospholipid unsaturation is a conserved feature as embryos develop to the blastocyst stage. Moreover, we show that lipid desaturases such as SCD1 are ...
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To explore the input limitations of microcentrifuge-based AP-MS workflows, we performed preliminary affinity purification experiments using transgenic HeLa Kyoto cell lines expressing SMC1A as a GFP fusion protein at near-endogenous levels using BAC TransgeneOmics17. SMC1A is a subunit of the human ...
Hepatocyte growth Employing integrated approaches, we defined metabolic drivers of invasive glioblastoma cells through the utilization of spatially addressable hydrogel biomaterial platforms, patient site-directed biopsies, and multi-omics analyses. The invasive borders of both hydrogel-cultured tumors and ...