et al. Low-input lipidomics reveals lipid metabolism remodelling during early mammalian embryo development. Nat Cell Biol 26, 278–293 (2024). https://doi.org/10.1038/s41556-023-01341-3 Download citation Received27 August 2022 Accepted20 December 2023 Published01 February 2024 Issue DateFebruary ...
Using low-input lipidomics in mouse and human embryos, Zhang, Shui, Li and colleagues find that lipid unsaturation increases with development towards the blastocyst stage. They further show that lipid desaturases contribute to successful embryo implantation.Zhang Ling...
www.nature.com/scientificreports OPEN A multi‑omics longitudinal study of the murine retinal response to chronic low‑dose irradiation and simulated microgravity Prachi Kothiyal 1*, Greg Eley 1, Hari Ilangovan 2, Katherine A. Hoadley 3, S. Robin Elgart 4...
Cold stress can lead to major crop losses. In natural ecosystems, there are several inputs of soil nitrogen. Even though our atmosphere consists of nearly 80% of N2, it is the limiting element in most ecosystems. Bio-available nitrogen can enter an ecosy
(v.2.1.2; bootstraps: 1000; model: GTR + I + G1) [103]. Phylogenetic trees were visualized with FigTree (v.1.4.4). Heatmap functions and statistical analysis to normalize input data, run clustering algorithm, and visualize the result with dendrograms were generated in RStudio...
According to manufacturer instructions, total RNA was extracted using RNA prep Pure Plant Kit (Tiangen Biotech-Beijing Co. Ltd). A volume of 1 μg RNA per sample was used as input material for the RNA sample preparation. Following the manufacturer's recommendations, sequencing libraries were gene...
Welti R, Shah J, Li W, Li M, Chen J, Burke JJ, et al. Plant lipidomics: Discerning biological function by profiling plant complex lipids using mass spectrometry. Front Biosci. 2002;12:2494–506. Article Google Scholar Download references ...
A total of 1 μg RNA per sample was used as input material to generate sequencing libraries using the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations, and index codes were added to attribute sequences to a specific sample. Briefly, ...
. We selected the 10 most significantly affected canonical pathways, as identified in the three analyses with proteomics alone, mRNA-seq alone, and combinatorial multiomics analysis. We then examined how using combinations of the individual omics results as input for the pathway analysis affected the...
complete loss ofSMPD1function causes childhood-onset NPD-A/B. Bearing in mind that ASM encoded bySMPD1catalyzes the conversion of sphingomyelin to phosphocholine and ceramide36, previous investigators observed significant differences in sphingomyelin and ceramide lipid species between postmortem brains ...