& Luo, H. Carbon limitation drives GC content evolution of a marine bacterium in an individual-based genome-scale model. ISME J. 12, 1180–1187 (2018). Article CAS PubMed PubMed Central Google Scholar Lynch, M. & Conery, J. S. The origins of genome complexity. Science 302, 1401–...
While many previous DNA storage encoding approaches included measures to exclude specific DNA patterns (homopolymers, GC content), we deliberately did not include such measures into our approach. Instead, our approach starts with the invertible pseudo randomization of the data, which elegantly avoids ...
H3K4me3 positive control locus TaqMan assay (probe with 5′ FAM and 3′ blackhole quencher 1) [RPL30-F CAAGGCAAAGCGAAATTGGT; RPL30-R GCCCGTTCAGTCTCTTCGATT; RPL30-P TCTCGCTAACAACTGCCCAGCTTTGAG], negative control locus SYBR Green assay [NegC1-F ACGTACCTTAAGCCCCTGGT; NegC1-R TAGTGCCTGG...
Ideally, primers should be 16-26 nucleotides in length, contain 50-60% guanines and cytosines (GC) and anneal to the matrix at approx= 55掳C (0-10掳C below the T_m: temperauire at which 50% of the DNA molecules are denatured; 2). The numerous protocols designed for the different...
To identify alterations in fatty acid content in the WAT of CAC patients, fatty acids were isolated from tissue samples and converted into FAMEs that were suitable for GC analysis. The content of palmitic acid (PA, C16:0) was significantly greater in the WAT of CAC patients. Similarly, the...
The average GC content and Q30 value was 46.37% and 93.44%, respectively, with 96.99% of clean reads successfully mapped to the reference genome. Moreover, these clean reads were assembled into 26,889 genes, with 2,967 identified as novel genes. To ensure the reliability and consistency of ...
The giant freshwater prawn (GFP) <italic>Macrobrachium rosenbergii</italic> is one of the important economically freshwater shrimp species, and its annual production in China is dominant in the world. However, its tolerance to low temperatures is extreme
Reverse transcription was performed using an iScript kit (Bio-Rad), and PCR was performed on a Bio-Rad SyberOne RT-qPCR thermocycler using primers as indicated in Table 2. Caspase activity Mice were anesthetized using isoflurane, and livers were perfused with 3 ml of ice-cold PBS. Whole ...
Medicinal plant microbiomes undergo selection due to secondary metabolite presence. Resident endophytic/epiphytic microorganisms directly influence plant’s bioactive compound synthesis. Hypothesizing low microbial diversity in Serjania erecta leaves, we
Yu JC, Hsu GC, Chen ST, Anton-Culver H, Ziogas A, Andrulis IL, Knight JA, Beesley J, Goode EL, Couch F, Chenevix-Trench G, Hoover RN, Ponder BA, Hunter DJ, Pharoah PD, Dunning AM, Chanock SJ, Easton DF (2009) Newly discovered breast cancer susceptibility loci on 3p24 and 17q23...