GC House / FCC Arquitectura Refurbishment of An Apartment from the 50s / Atelier Starzak Strebicki Seven Degrees Restaurant / Kursimerah Studio THE YELLOW BOX / TRAANSPACE Shenzhen Tsinghua University Graduate School Innovation Center / CAPOL Makomanai Church United Church of Christ in Japa...
We noticed brief and sudden spikes in force-clamp traces where the force momentarily drops with a spike duration of ~0.16 ± 0.01 s. These spikes mark the corresponding extensions of tip-linkcomplexesunder tension, which can be quantified from the sudden jumps in the tip-surface distances...
All primers were chosen to be as close to 20 bp and 50% GC content as possible to maintain consistent annealing temperatures between constructions (58°C for fragment amplifications, 55°C for multi-fragment splicing amplifications). Efforts were made to avoid repetitive regions, GC runs, hair...