Choose a function: Select ANALYZE for easy, one-click access to a Tmcalculator, GC content calculator, extinction coefficient calculator, and more. Predict oligo secondary structure or possible duplexes with HAIRPIN, SELF-DIMER, and HETERO-DIMER options. ...
Primers are for PCR and sequencing should have GC content between 40 – 60%, with the 3’ end of the primer ending C or G to promote binding by DNA polymerase. In the last 5 bases at the 3’ end, there should be at least 2 G or C bases — this is known as a GC Clamp. G...
If the Tm of your primer is very low, try to find a sequence with more GC content, or extend the length of the primer a little. Aim for the GC content to be between 40 and 60%, with the 3′ of a prim...
We are pleased to offer OligoEvaluator™, our online oligonucleotide sequence calculator that provides primer analysis values for PCR: Base count Molecular weight Extinction coefficient Oligo type µg/OD at 260 nm Length (base pairs) Primer melting temperature Tm (°C) GC ...
SBayesR: https://cnsgenomics.com/software/gctb/#Overview SMARTPCA: https://github.com/chrchang/eigensoft/wiki/smartpca SMR: https://cnsgenomics.com/software/smr/#Overview SNP2HLA: http://software.broadinstitute.org/mpg/snp2hla/ SNPTEST: https://mathgen.stats.ox.ac.uk/genetics_software/snpt...
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SBayesR: https://cnsgenomics.com/software/gctb/#Overview SMARTPCA: https://github.com/chrchang/eigensoft/wiki/smartpca SMR: https://cnsgenomics.com/software/smr/#Overview SNP2HLA: http://software.broadinstitute.org/mpg/snp2hla/ SNPTEST: https://mathgen.stats.ox.ac.uk/genetics_software/snpt...
Select ANALYZE for easy, one-click access to a Tm calculator, GC content calculator, extinction coefficient calculator, and more. Predict oligo secondary structure or possible duplexes with HAIRPIN, SELF-DIMER, and HETERO-DIMER options. Search for matches to your input oligo sequence in the NCBI...
SBayesR: https://cnsgenomics.com/software/gctb/#Overview SMARTPCA: https://github.com/chrchang/eigensoft/wiki/smartpca SMR: https://cnsgenomics.com/software/smr/#Overview SNP2HLA: http://software.broadinstitute.org/mpg/snp2hla/ SNPTEST: https://mathgen.stats.ox.ac.uk/genetics_software/snpt...
Primers are preferably 22-30 nucleotides in length, have a GC content of at least 50% and anneal to the target sequence at temperatures of about 68° C. to 72° C. The amplified region may be sequenced as described above, and overlapping sequences assembled into a contiguous sequence. ...