Choose a function: Select ANALYZE for easy, one-click access to a Tmcalculator, GC content calculator, extinction coefficient calculator, and more. Predict oligo secondary structure or possible duplexes with HAIRPIN, SELF-DIMER, and HETERO-DIMER options. ...
The default value = 25.0 °C (298K). How do you calculate the annealing temperature of a primer? The OligoEvaluator™ provides melting temperature for oligos. Just enter you sequence in the calculator Analysis module, and you'll find the Tmreported in the seventh column. ...
Primer GC: This is the minimum and maxiumum per- centage of guanine and cytosine (GC) allowed. The GC content of primers is used to determine the melting tem- perature of the primer, which can be used to predict the annealing temperature. The melting temperature of pri- mers is ...
GC content >>> primer1.gc_content 0.5217391304347826 GC clamp >>> primer1.gc_clamp 1 Single run length >>> primer1.single_runs {'A': 2, 'T': 1, 'C': 1, 'G': 2} Double run length >>> primer1.double_runs {'TA': 1, 'TC': 0, 'TG': 1, 'AT': 0, 'AC': 1, 'AG...
SBayesR: https://cnsgenomics.com/software/gctb/#Overview SMARTPCA: https://github.com/chrchang/eigensoft/wiki/smartpca SMR: https://cnsgenomics.com/software/smr/#Overview SNP2HLA: http://software.broadinstitute.org/mpg/snp2hla/ SNPTEST: https://mathgen.stats.ox.ac.uk/genetics_software/snpt...
SBayesR: https://cnsgenomics.com/software/gctb/#Overview SMARTPCA: https://github.com/chrchang/eigensoft/wiki/smartpca SMR: https://cnsgenomics.com/software/smr/#Overview SNP2HLA: http://software.broadinstitute.org/mpg/snp2hla/ SNPTEST: https://mathgen.stats.ox.ac.uk/genetics_software/snpt...
1). The input nucleotide sequence ‘TCAGACCCATTAAAAAGCGCCGACGT’ of a DNA primer of 26 nt in length and carrying a desired mutation is provided in the input window having the header ‘Mutant Primer’. The ‘SUBMIT’ button below the input window starts the analysis by REMP. As a first ...
Primer-specific characteristics were calculated including the GC-content, basic and salt adjusted melting temperature (Tm) using the program OligoCalc [70]. Positions of the primers were referenced to the 18S rRNA gene sequence of Saccharomyces cerevisiae (acc. No. Z75578, [71]). Finally primer...
with a slight overrepresentation of thymine, which may be caused either by bias within the ordered primer mixtures or also possible due to altered template binding behavior within the primers containing a higher GC content due to more pronounced secondary structure occurrence, leading to less efficie...
Choose a function: Select ANALYZE for easy, one-click access to a Tmcalculator, GC content calculator, extinction coefficient calculator, and more. Predict oligo secondary structure or possible duplexes with HAIRPIN, SELF-DIMER, and HETERO-DIMER options. ...