本课题通过细胞功能实验,组织标本检测及体内实验探索Lnc RNA-ROR调节HCC细胞低氧诱导自噬的具体机制,从而为HCC的临床诊治提供新的方向,思路与理论基础.研究方法:1.(1)低氧处理HCC细胞,应用免疫印迹实验(Western blot)检测LC3,P62蛋白表达,免疫荧光检测LC3表达,透射电镜观察自噬小体;(2)应用生物信息技术筛选低氧相关Lnc...
目的探讨长链非编码RNA ROR(ROR)和长链非编码RNA ATB(ATB)在膀胱癌患者中的表达情况和临床意义。方法选取在重庆市开州区人民医院泌尿外科住院的60例膀胱癌患者和同期60例健康者为研究对象。采用实时荧光定量PCR检测膀胱癌患者癌组织、癌旁组...
In the current study, we focused on the expression changes and clinical values of lncRNA ROR and miR-145-5p in OP clinical serum samples, and investigated the interactive modulation effect of ROR/miR-145-5p on osteoblast function. Serum samples were obtained from 82 OP patients and 79 ...
Therefore, the article “Mechanism of LncRNA ROR promoting prostate cancer by regulating Akt, by X.-Q. Zhai, F.-M. Meng, S.-F. Hu, P. Sun, W. Xu, published in Eur Rev Med Pharmacol Sci 2019; 23 (5): 1969-1977–DOI: 10.26355/eurrev_201903_17235–PMID: 30915739” has been ...
摘要: 目的 观察子宫内膜癌患者血清中中长链非编码RNA(LncRNA)重编程调节物(ROR)、微小RNA(miR)-29a的表达变化,并探讨其临床意义.方法 选取85例子宫内膜癌患者作为癌症组,以同期40例子宫肌瘤患者为对照组.应用实时荧光定量PCR法检测两组血清中LncRNA ROR、miR-29a表达,分析LncRNA ... 查看全部>> ...
五、探索lncrna MAYA是否以依赖NSUN6的方式抑制MST1的甲基化从而激活MST1的磷酸化,确定ROR1/HER3-LLGL2-MAYA-NSUN6信号轴之间的串扰。 六、通过乳腺癌骨转移模型发现shRNA诱导敲除MAYA减少了小鼠肿瘤负担,抑制了乳腺癌的骨转移,证明了通过抑制lncrna MAYA来抑制乳腺癌骨转移的有效性。
Functional experiments presented that lncRNA ROR silence mitigated H/R-stimulated inflammatory damage, oxidative stress, and ferroptosis in AC16 cells, whereas these roles could be reversed by co-downregulation of miR-769-5p or co-overexpression of CBX7. These data uncovered that lncRNA ROR ...
Besides, lncRNA-ROR significantly down-regulated the miR-124-3p expression and miR-124-3p exposure inhibited the protecting impact of lncRNA-ROR on LPS-induced C28/12 cells. Furthermore, lncRNA-ROR suppressed the TLR4/Myd88/NF-魏B pathways via suppressing the expression of miR-...
Long Non‐Coding RNA Reprogramming (lncRNA‐ROR) plays an important role in regulating various biologic processes, whereas the effect of lncRNA‐ROR in osteoarthritis (OA) is little studied. This study aimed to explore lncRNA‐ROR expression in articular cartilage and identify the functional ...
LncRNA-RORmiR-135a-5pIschemic stroke is a common cerebrovascular disease with high morbidity, disability and mortality. LncRNAs were involved in ischemia/reperfusion injury. The present study aims to investigate whether lncRNA ROR can promote the cerebral hypoxia/reoxygenation (H/R) injury in vitro...