本研究对lincRNA-ROR在肝纤维化中的作用机制进行了深入的探究。实验结果表明,lincRNA-ROR的表达水平在肝纤维化病变组织中显著升高。通过建立肝纤维化模型,我们发现lincRNA-ROR通过miR-145/ZEB2调控HERC5介导的p53蛋白ISG化修饰在肝纤维化中发挥了重要作用。这为肝纤维化的治疗提供了新的分子靶点和治疗策略。 关键词...
linc-RoRmiR-145Endometrial cancerTumorsphereObjectives. Recently, large intergenic non-coding ribonucleic acids-RoR (linc-RoR) was reported to regulate expression of core stem cell transcription factors (TFs), but its role in endometrial tumorsphere is still unknown. Methods. Fluorescence in situ ...
Nanog mRNA表达采用RT-qPCR技术检测诱导生成的OCS-LC中Linc-ROR mRNA较诱导前A2780贴壁细胞的表达差异,si-OCS-LC和control-OCS-LC细胞球中核心转录因子Oct-4、Nanog mRNA表达水平,以及Linc-ROR RNA、miR-181c-5p RNA的变化情况。
目的:探讨基因间长链非编码RNA-重编码调控因子(Linc-ROR RNA)在卵巢癌干细胞样细胞(OCS-LC)干性调控及功能维持中的作用及其调控机制.方法:采用无血清悬浮培养条件诱导A2780细胞生成OCS-LC,通过成球实验,分化实验以及流式分析技术分析CD133^(+)细胞率,鉴定细胞干性特征.生物信息学分析,确定Linc-ROR RNA与miR-181c...
However, little is known about the role of LincRNA-ROR (linc-ROR) in PC. In the present study, we found that linc-ROR was upregulated in PC tissues. Overexpression of linc-ROR promoted cells proliferation, migration, invasion and metastasis both in vitro and in a mouse model. Contrarily,...
The long intergenic non-coding RNA, regulator of reprogramming (linc-ROR) is an oncogene and plays a key role in the embryonic stem cell maintenance and is involved in cancer progression. The objective of this study was to analyze linc-ROR expression in
Highlights ► A lincRNA-RoR function as a key competing endogenous RNA in hESCs ► Linc-RoR prevents core TFs from microRNA-mediated suppression ► Linc-RoR forms a feedback loop with core TFs and microRNAs ► Linc-RoR is a key regulator of hESC self-renewal and differentiationPrevious...
The inhibition of lincRNA‐ROR (ROR) downregulated HERC5 expression and elevated p53 expression in TGF‐β1‐stimulated LX‐2 cells. Furthermore, the expression of p53 was almost unchanged after TGF‐β1‐stimulated LX‐2 cells were co‐transfected with a ROR‐expressing plasmid ...
长链非编码RNA Linc-ROR与恶性肿瘤发生,发展关系的研究进展刘帅臣李玉明
目的探讨长链非编码RNA(lincRNA)-ROR作为一个内源竞争RNA(ceRNA)结合miR-145对下游干性相关基因Oct4、Sox2和Nanog的表达,及对结直肠癌干细胞生物学特性的影响,并阐明这种分子调控网络的临床意义。方法收集2014年至2016年间河南省南阳市中心医院及南阳市第二人民医院胃肠外科结直肠癌患者的癌组织及癌旁组织共52例,...