Fast formation and maturation enhancement of human liver organoids using a liver-organoid-on-a-chipdoi:10.3389/fcell.2024.1452485Jae Hee ByeonDa Jung JungHyo-Jeong HanWoo-Chan SonGi Seok JeongFrontiers in Cell & Developmental Biology
The liver is the most important metabolic organ in the body. While mouse models and cell lines have further deepened our understanding of liver biology and related diseases, they are flawed in replicating key aspects of human liver tissue, particularly i
Liver organoids of different sizes, shapes and cell compositions have been reported and could be broadly classified into several categories: 1) Stem cell and progenitor organoids which consist of proliferative cells that can be expanded in scale and differentiated to liver cell types; 2) Pluripotent...
Organoid-on-a-chip and body-on-a-chip systems for drug screening and disease modeling Drug Discovery Today (2016) W.L.K. Chen et al. Integrated gut/liver microphysiological systems elucidates inflammatory inter-tissue crosstalk Biotechnology and Bioengineering (2017) A. Choe et al. Microfluidic ...
The proposed human NAFLD-on-a-chip model combines stem cell organoids with organs-on-chips, which may provide a promising platform for extending their applications for disease studies and effective therapies. 展开 关键词: organoids-on-a-chip liver organoid human-induced pluripotent stem cells ...
Liver organoids Organoids contain a population of self-renewing stem cells that share a spatial organization similar to that of their corresponding organs and are capable of reproducing partial functions, thereby providing a relevant pathophysiological system [111]. In contrast, spheroids are usually fo...
Interestingly, this approach combined with human liver organoids to form a liver-on-chip system with high-throughput characteristics, and therefore provides a potential platform for DILI risk assessment [85]. Single-cell proteomics of the peripheral blood mononuclear cells from biological samples ...
Based on the drug discovery platform, the liver-on-chip segment is expected to grow at the highest rate during the forecast period. Based on the drug discovery platform, the market will be divided into liver organoids, liver-on-chip, 2D models, animal models, and 3D bioprinting. The liver...
Culture of progenitor cells has been used to study the dynamics of their growth into organoids. One successful culture of mice Lgr5+ cells (actively dividing stem cells) from damaged liver has been reported by Hans Clevers’ group [146]. These organoids can be differentiated into cells ...
Purification methods can be based on primary tissues or organoids. For primary tissues, flow sorting or magnetic bead sorting can be performed before organoid culture to improve the purity of tumor cells, and subsequently perform tumor organoid culture. Alternatively, the digestion time can be increa...