Fast formation and maturation enhancement of human liver organoids using a liver-organoid-on-a-chipdoi:10.3389/fcell.2024.1452485Jae Hee ByeonDa Jung JungHyo-Jeong HanWoo-Chan SonGi Seok JeongFrontiers in Cell & Developmental Biology
organoids as a three-dimensional structure derived from (pluripotent) stem cells, progenitor, and/or differentiated cells that self-organize through cell-cell and cell-matrix interactions to recapitulate aspects of the native tissue architecture and function in vitro [8]. Although early reports on th...
These HLCs are integrated into various culture systems including 2D culture, 3D organoids or spheroids, bioprinted tissue constructs, and liver-on-a-chip (microfluidic) devices. The culture systems can then be used to model various liver diseases including HBV, HCV, malaria, NAFLD, ALD, and ...
Liver organoids of different sizes, shapes and cell compositions have been reported and could be broadly classified into several categories: 1) Stem cell and progenitor organoids which consist of proliferative cells that can be expanded in scale and differentiated to liver cell types; 2) Pluripotent...
Future advancements may position HLOs as a standard in vitro model for compound toxicity screening. Some drawbacks of HLOs, including limited maturity and functionality, as well as challenges in controlling heterogeneity [119], which are indeed also present in liver organoids, are expected to be gr...
Future advancements may position HLOs as a standard in vitro model for compound toxicity screening. Some drawbacks of HLOs, including limited maturity and functionality, as well as challenges in controlling heterogeneity [119], which are indeed also present in liver organoids, are expected to be gr...
For example, cholangiocytes express the KRT19, KRT7, and SOX9 genes, while hepatocytes express the ALB, HNF4A, and MRP4 genes [31]. Finally, the maturity of the structural organization of the organoids can be confirmed via magnification, and depending on the culture system, the morphology...
They had to overcome the different issues of the scaffold-free culture approaches, like the lack of zonation effect in long-term liver organoid culture, or the questioned physiological relevance of the extracellular matrix (ECM). The idea behind their device was to bypass the need for a ...
A. Skardal et al. Organoid-on-a-chip and body-on-a-chip systems for drug screening and disease modeling Drug Discovery Today (2016) W.L.K. Chen et al. Integrated gut/liver microphysiological systems elucidates inflammatory inter-tissue crosstalk Biotechnology and Bioengineering (2017) A. Choe...
Au et al. prepared a liver-on-a-chip platform via digital microfluidic technology to evaluate individual 3D liver organoids’ responsiveness to drugs [26]. Nevertheless, traditional digital microfluidics platforms can only perform multiplex or parallel biochemical reactions due to the use of a small...