Fast formation and maturation enhancement of human liver organoids using a liver-organoid-on-a-chipdoi:10.3389/fcell.2024.1452485Jae Hee ByeonDa Jung JungHyo-Jeong HanWoo-Chan SonGi Seok JeongFrontiers in Cell & Developmental Biology
Herein, we discuss these biologically engineered organoids and microphysiological systems. Introduction The purpose of this review is to inform the reader of the significance and available methods for replicating human liver physiology and pathology ex vivo. First, the physiologic roles of the liver ...
These HLCs are integrated into various culture systems including 2D culture, 3D organoids or spheroids, bioprinted tissue constructs, and liver-on-a-chip (microfluidic) devices. The culture systems can then be used to model various liver diseases including HBV, HCV, malaria, NAFLD, ALD, and ...
organoids as a three-dimensional structure derived from (pluripotent) stem cells, progenitor, and/or differentiated cells that self-organize through cell-cell and cell-matrix interactions to recapitulate aspects of the native tissue architecture and function in vitro [8]. Although early reports on th...
s complexity. This review highlights recent progress in liver organoid research, with a particular emphasis on their potential for toxicity assessment and disease modeling. The intrinsic advantages of liver organoids include higher sensitivity and suitability for long-term studies, which enhance the ...
s complexity. This review highlights recent progress in liver organoid research, with a particular emphasis on their potential for toxicity assessment and disease modeling. The intrinsic advantages of liver organoids include higher sensitivity and suitability for long-term studies, which enhance the ...
organoid technology pioneered by the Clever’s group has been harnessed to successfully expand patient-derived material (Sato et al.2009). Using a cocktail of growth factors and small molecules, the group was able to derive LGR5+adult stem cells from small intestine tissues, which expand as sph...
The global human liver models market can be segmented based on platform type and region. In terms of platform type, the market can segmented into liver organoids, animal models, 2D models, liver-on-a-chip, and 3D bio-printed models. As animal models...
Future advancements may position HLOs as a standard in vitro model for compound toxicity screening. Some drawbacks of HLOs, including limited maturity and functionality, as well as challenges in controlling heterogeneity [119], which are indeed also present in liver organoids, are expected to be gr...
The present study aimed to develop viable liver organoids using decellularized native liver scaffolds and evaluate the efficacy of human liver organoid transplantation in a rabbit model of cirrhosis. Liver organoids were formed by coculture of hepatocyte-like cells derived from the human-induced plurip...